南方农业学报2025，Vol.56Issue(4)：1070-1079,10.DOI:10.3969/j.issn.2095-1191.2025.04.006
1株野生革耳分子鉴定、生物学特性分析及驯化栽培
Molecular identification,biological characteristics analysis,and domestication cultivation of a wild Panus rudis strain
摘要
Abstract
[Objective]Molecular identification,biological characteristics analysis and artificial domestication and cul-tivation of a wild species of Panus rudis were conducted to provide theoretical references for the development and utiliza-tion of wild Panus rudis and the breeding of superior varieties.[Method]Pure culture strains were obtained by tissue isola-tion from the fruiting bodies of the wild Panus rudis strain D559 collected from the Mongolian Plateau.The classification and identification were carried out by combining biological characteristics with the analysis of the internal transcribed spacer(ITS)sequence.Single-factor experiments were conducted on carbon sources,nitrogen sources,pH and tempera-ture,and 3 better levels were selected for orthogonal experiments.Finally,artificial domestication and cultivation of this strain were carried out.[Result]The fruiting body of wild Panus rudis had a cap diameter of 3 to 10 cm,funnel-shaped,with a surface covered in fine hairs.The gills were white when fresh and turned light yellow when dry,narrow and dense.The stipe was 1 to 2 cm long and 0.3 to 1.0 cm in diameter,cylindrical or with a slightly enlarged base,lateral to eccentric,fibrous and solid.The spore print was white,the spores were smooth,colorless and ellipsoidal(6-8 μm×3-4 μm).The cystidia were cylindrical to clavate(45-50 μm×10-13 μm).The strain was identified as Panus rudis based on ITS se-quence comparison results.The single-factor experiment results showed that the optimal carbon source,nitrogen source,pH and temperature for this strain were starch,yeast extract,4.0 and 30℃respectively.The orthogonal experiment results indicated that the best mycelial growth was achieved under the conditions of dextrin as carbon source,beef extract as nitro-gen source,pH 5.0 and temperature 35℃.The order of influence of the 4 factors on the mycelial growth of Panus rudis was:temperature>carbon source>pH>nitrogen source.The suitable cultivation formula was 78%coarsely chopped wood chips,20%wheat bran,1%lime and 1%gypsum,with a moisture content of 55%to 60%.Under dark conditions at 30℃,it took 26 to 35 d for the mycelium to fill the bag,with a mycelial growth rate of 3.75 to 4.22 mm/d.The fruiting bodies matured in about 20 d at a temperature of 18 to 25℃and a relative humidity of 80%to 90%.[Conclusion]The wild strain D559 of Panus rudis is a thermophilic and acidophilic fungus.Its mycelium grows well in a medium with dex-trin as the carbon source and beef extract as the nitrogen source,at pH 5.0 and a temperature of 35℃.It has a short artifi-cial cultivation cycle and great development potential,and thus has extensive application value.关键词
野生革耳/生物学特性/单因素试验/正交试验/驯化栽培Key words
wild Panus rudis/biological characteristics/single-factor experiment/orthogonal test/domestication cultivation分类
农业科技引用本文复制引用
戴建成,戴丹,胡佳,陈绪涛,彭新红,孙鹏,王振,魏云辉..1株野生革耳分子鉴定、生物学特性分析及驯化栽培[J].南方农业学报,2025,56(4):1070-1079,10.基金项目
江西省技术创新引导类科技计划科技合作专项(20212BDH80009) （20212BDH80009）
江西省重点研发计划项目(20212BBF61002) （20212BBF61002）
江西现代农业科研协同创新专项(JXXTCXBSJJ202212) Jiangxi Technology Innovation Guidance Science and Technology Plan Science and Technology Cooperation Special Project(20212BDH80009) （JXXTCXBSJJ202212）
Jiangxi Key Research and Development Plan Project(20212BBF61 002) （20212BBF61 002）
Jiangxi Modern Agricultural Scientific Research Collaborative Innovation Special Project(JXXTCXBSJJ202212) （JXXTCXBSJJ202212）
