南方农业学报2025，Vol.56Issue(4)：1112-1123,12.DOI:10.3969/j.issn.2095-1191.2025.04.010
橡胶林下中药材根系内生真菌多样性及其与根际土壤理化性质的相关分析
Diversity of endophytic fungi in the roots of traditional Chinese medicines under rubber forests and their correlation with the physicochemical properties of rhizosphere soil
摘要
Abstract
[Objective]To analyze the diversity of endophytic fungi in the roots of traditional Chinese medicines under rubber plantations and their correlation with the physicochemical properties of the rhizosphere soil,which could provide theoretical basis for the cultivation of traditional Chinese medicines and the development and utilization of endophytic fun-gal resources in the roots.[Method]Baphicacanthus cusia,Amomum villosum,Alpinia oxyphylla and Alpinia officinarum root and rhizosphere soil samples of 4 traditional Chinese medicines were all collected from the demonstration base in Pu-wen Town,Jinghong City,Xishuangbanna Dai Autonomous Prefecture,Yunnan Province.The ITS sequences of endo-phytic fungi in the roots were sequenced to analyze the diversity of endophytic fungal communities in the roots.Linear dis-criminant analysis effect size(LEfSe)was used to screen the species with significant differences among the endophytic fungal communities in the roots of 4 traditional Chinese medicines.The physicochemical properties of the rhizosphere soil were determined and its correlation with the endophytic fungal community of the root was analyzed.[Result]The pH of the rhizosphere soil of Baphicacanthus cusia was significantly lower than that of Alpinia officinarum(P<0.05,the same below),and the content of available phosphorus was significantly higher than that of Amomum villosum,Alpinia oxy-phylla and Alpinia officinarum.The total potassium content in the rhizosphere soil of Baphicacanthus cusia,Amomum vil-losum and Alpinia officinarum was significantly higher than that of Alpinia oxyphylla.The Chao1 index of the endophytic fungal communities in the roots of Baphicacanthus cusia,Amomum villosum and Alpinia officinarum was significantly higher than that of Alpinia oxyphylla,indicating that the abundance of the endophytic fungal communities in the roots of Baphicacanthus cusia,Amomum villosum and Alpinia officinarum was significantly higher than that of Alpinia oxyphylla.A total of 8 phyla were detected in the endophytic fungal communities of the roots of 4 traditional Chinese medicines.The dominant phyla of Baphicacanthus cusia,Amomum villosum and Alpinia officinarum were Ascomycota and Basidiomy-cota,while the dominant phylum of Alpinia oxyphylla was Ascomycota.Through LEfSe analysis,it was found that the en-dophytic fungal communities in the roots of 4 traditional Chinese medicines all had biomarkers that could distinguish them from each other.The correlation analysis results showed that in the endophytic fungal community of Amomum villosum,the Glomeromycota phylum was significantly and positively correlated with the organic matter content in the rhizosphere soil.In the endophytic fungal community of the roots of Alpinia oxyphylla,Glomeromycota was extremely significantly and negatively correlated with the total potassium content(P<0.01).The Chao1 index of the endophytic fungal community in the roots of Amomum villosum was significantly and positively correlated with the pH of the rhizosphere soil.The Shan-non index of the endophytic fungal community in the roots of Alpinia officinarum was significantly and negatively corre-lated with pH of the rhizosphere soil,while the Shannon index of the endophytic fungal community in the roots of the other 3 Chinese herbal medicines had no significant correlation with the physicochemical properties of the rhizosphere soil(P>0.05).[Conclusion]All 4 traditional Chinese medicines have abundant endophytic fungal resources in their roots.The diversity of endophytic fungi in the roots of Baphicacanthus cusia,Amomum villosum and Alpinia oxyphylla has no sig-nificant correlation with the physicochemical properties of the rhizosphere soil.In contrast,the diversity of endophytic fungal communities in the roots of Alpinia officinarum responds more strongly to the pH changes of the rhizosphere soil.关键词
中药材/根际土壤/内生真菌/群落组成/多样性Key words
traditional Chinese medicines/rhizosphere soil/endophytic fungi/community composition/diversity分类
农业科技引用本文复制引用
杞美军,葛宇,宋德晶,董诗浪,王步天,杜华波,曲鹏,普天华,施学东,吕维香..橡胶林下中药材根系内生真菌多样性及其与根际土壤理化性质的相关分析[J].南方农业学报,2025,56(4):1112-1123,12.基金项目
云南省专家工作站项目(202305AF150125) （202305AF150125）
国家天然橡胶产业技术体系普洱综合试验站项目(CARS-33-YN4) （CARS-33-YN4）
云南省科技特派队项目(202304BI090032-58) （202304BI090032-58）
云南农业大学本科实践教学、劳动教育和耕读教育教学改革研究与实践项目(2024SJJX036) Yunnan Expert Workstation Project(202305AF150125) （2024SJJX036）
Pu'er Comprehensive Experimental Station of the National Natural Rubber Industry Technology System(CARS-33-YN4) （CARS-33-YN4）
Yunnan Science and Technology Special Dispatch Team Project(202304BI090032-58) （202304BI090032-58）
Yunnan Agricultural University Undergraduate Practice Teaching,Labor Education and Farming Education Teaching Reform Research and Practice Project(2024SJJX036) （2024SJJX036）
