南方农业学报2025，Vol.56Issue(4)：1147-1157,11.DOI:10.3969/j.issn.2095-1191.2025.04.013
野蛞蝓响应茶皂素胁迫的转录组分析
Transcriptome analysis of Agriolimax agrestis in response to tea saponin stress
摘要
Abstract
[Objective]To screen the genes related to detoxification metabolism of Agriolimax agrestis under tea sapo-nin stress,and explore the resistance mechanism of Agriolimax agrostis to biological pesticides,which could provide scien-tific basis for the study of drug resistance mechanism and drug resistance management of Agriolimax agrostis.[Method]In this study,Illumina high-throughput sequencing technology was used to sequence the transcriptome(RNA-Seq)of Agriolimax agrestis in tea saponin and blank control treatments.The functional annotation,functional classification and metabolic pathway analysis of differentially expressed genes(DEGs)were performed using bioinformatics softwares such as Trinity,DESeq2 and GOseq,and the screened drug-resistant related genes were verified by real-time fluorescence quantitative PCR.[Result]The results showed that 1359 DEGs were screened from the transcriptome data,including 586 significantly up-regulated genes(P<0.05,the same below),773 significantly down-regulated genes.A total of 51 DEGs related to insect drug resistance were screened,involving metabolic resistance genes such as glutathione S-transferase(GST),cytochrome P450(CYP450)and carboxylesterase(CarE).GO functional annotation analysis showed that DEGs were mainly related to oxidoreductase activity,transcription of DNA templates,organelle pathways and DNA binding.A total of 232 DEGs were mapped to 129 different metabolic pathways in KEGG,mainly involving in antigen processing and presentation,protein processing fluid shear stress in endoplasmic reticulum and atherosclerosis,drug metabolism-cytochrome P450 and other metabolic pathways closely related to insect resistance.The results of real-time fluorescence quantitative PCR of drug resistance related genes were consistent with those of transcriptome,which in-dicated that the sequencing data of transcriptome was accurate and reliable.[Conclusion]The Agriolimax agrestis tea sapo-nin stress are analyzed by transcriptome sequencing technology,and detoxification metabolism-related genes such as GST,cytochrome P450 and CarE are mined.These genes play a key role in the stress response of Agriolimax agrestis when facing the threat of tea saponin and are the core factors of their drug resistance.关键词
野蛞蝓/转录组/茶皂素/抗药性Key words
Agriolimax agrestis/transcriptome/tea saponin/drug resistance分类
农业科技引用本文复制引用
王艺焜,祖庆学,祝紫江,杨超,李发银,刘杰,冯裕洋,张长禹..野蛞蝓响应茶皂素胁迫的转录组分析[J].南方农业学报,2025,56(4):1147-1157,11.基金项目
国家重点研发计划项目(2023YFD1400700) （2023YFD1400700）
贵州省烟草公司贵阳市公司科技项目(2022-07) National Key Research and Development Program of China(2023YFD1400700) （2022-07）
Science and Technology Project of Guiyang Company of Guizhou Tobacco Company(2022-07) （2022-07）
