南方农业学报2025，Vol.56Issue(4)：1171-1181,11.DOI:10.3969/j.issn.2095-1191.2025.04.015
湖北省生姜白星病病原菌鉴定与室内药剂筛选
Pathogen identification and indoor fungicides screening for white scab disease of ginger(Zingiber officinale)in Hubei Province
摘要
Abstract
[Objective]To study the species and biological characteristics of the pathogen causing ginger(Zingiber of-ficinale)white scab disease,and to screen effective fungicides for inhibiting mycelium growth of the pathogen in the labo-ratory,which could provide basis for field control of ginger white scab disease.[Method]Diseased ginger leaves were col-lected from a ginger plantation in Laifeng County,Enshi Prefecture,Hubei Province.The pathogen was isolated and puri-fied by tissue separation method,and pathogenicity was assessed based on Koch's rule.Sequences of ITS,EF-1α,ACT,CAL,TUB2,and GAPDH of the pathogen were amplified using specific primers.The pathogen was identified by combin-ing morphological characteristics and molecular identification.The biological characteristics of the pathogen and the in-hibitory effects of 8 commonly used fungicides on mycelium growth were evaluated using the mycelium growth rate method.[Result]Five strains of pathogens with consistent morphology were isolated and purified from the diseased ginger leaves.The representative strain 23-5 was selected for morphological and molecular biological identification.Its morpho-logical characteristics were consistent with those of Neoscirrhia matteucciicola;after comparative analysis,the reference strain sequences with high coverage and similarity were selected.The phylogenetic tree was constructed after splicing the ITS-TUB2 sequence.The results showed that strain 23-5 and Neoscirrhia matteucciicola were clustered in the same branch.The pathogen of ginger white scab disease was determined to be Neoscirrhia matteucciicola.The determination results of the biological characteristics of the pathogen indicated that the lethal temperature of the pathogen was 54℃;the mycelium growth was more suitable at pH of 6 to 8.When the pH was 7,the colony diameter was the largest,reaching 6.45 cm.The optimal carbon source for pathogen was soluble starch,and the suitable nitrogen sources were peptone,yeast extract and beef extract powder.Among the 8 tested fungicides,difenoconazole had the best inhibitory effect on the mycelium growth of strain 23-5,with an inhibitory medium concentration(EC50)of 0.05 mg/L.[Conclusion]This study is the first to identify Neoscirrhia matteucciicola as the pathogen of ginger white scab disease in Hubei Province.Difeno-conazole demonstrates a good inhibitory effect on the mycelium growth of this pathogen and can be recommended as a candidate fungicide for field control of ginger white scab disease.关键词
生姜/白星病/Neoscirrhia matteucciicola/药剂筛选/湖北省Key words
ginger(Zingiber officinale)/white scab disease/Neoscirrhia matteucciicola/fungicides screening/Hubei Provincce分类
农业科技引用本文复制引用
周洁,夏良荣,邱俊杰,齐传东,徐志超,郭凤领,吴金平..湖北省生姜白星病病原菌鉴定与室内药剂筛选[J].南方农业学报,2025,56(4):1171-1181,11.基金项目
国家重点研发计划项目(2021YFD1600300) （2021YFD1600300）
国家特色蔬菜产业技术体系项目(CARS-24-G-17) （CARS-24-G-17）
湖北省农业科技创新中心创新团队项目(2024-620-000-001-007) National Key Research and Development Program of China(2021YFD1600300) （2024-620-000-001-007）
China Agricul-ture Research System—Featured Vegetable(CARS-24-G-17) （CARS-24-G-17）
Hubei Agricultural Science and Technology Innovation Center Innovation Team Project(2024-620-000-001-007) （2024-620-000-001-007）
