南方农业学报2025，Vol.56Issue(4)：1182-1192,11.DOI:10.3969/j.issn.2095-1191.2025.04.016
光氮互作对赤苍藤苗木叶片解剖结构的影响
Effects of light-nitrogen interaction on leaf anatomical structure of Erythropalum scandens seedlings
摘要
Abstract
[Objective]To explore the effects of light,nitrogen fertilizer and their interaction on the leaf structure of Erythropalum scanden cuttings,which could provide scientific basis for the study of the ratio of light and nitrogen suita-ble for the growth and development of Erythropalum scanden leaves and the cultivation of strong seedlings of Erythro-palum scanden.[Method]Taking the cuttings of 2-year-old Erythropalum scanden as the research object,4 light transmit-tance levels[100.00%natural light(NS)(L0),70.85%NS(L1),52.45%NS(L2),13.85%NS(L3)]and 5 nitrogen levels(0,2.5,5.0,7.5 and 10.0 g/plant urea)were set,marked as N0,N1,N2,N3 and N4 respectively,with 100.00%NS and 0 g/plant urea as the control(L0N0),there were a total of 20 combination treatments of light and nitrogen.Leaf anatomical structures of Erythropalum scandens under different treatments were observed to analyze and compare the ef-fects on leaf thickness,cuticle thickness,epidermal thickness,and mesophyll structure.A comprehensive analysis of the impacts of light-nitrogen interactions on leaf anatomy was conducted.[Result]The leaf tissue structure of Erythropalum scandens was arranged sequentially as follows:upper cuticle,upper epidermis,palisade tissue,spongy tissue,lower epi-dermis and lower cuticle.The main effect of light had extremely significant impact on leaf thickness of Erythropalum scan-dens(P<0.01,the same below)while the main effect of nitrogen significantly affected leaf thickness(P<0.05,the same below).The highest leaf thickness of Erythropalum scandens(114.20 μm)was observed in L0N0,which was extremely significantly higher than all nitrogen treatments under L2 and L3.Under the same nitrogen level,leaf thickness and upper cuticle thickness generally decreased with reduced light intensity.Moreover,the L0 full light treatment was greater than other light treatments,the thickness of the spongy tissue initially decreased and then increased.Under the same light inten-sity,with the increase of nitrogen level,lower epidermal thickness first increased and then decreased with increasing ni-trogen levels in L0 and L1 light environments.Palisade tissue thickness under L0 and L1 light environments also followed the changing trend of increasing first and then decreasing,while no significant changes were observed under L2 and L3 light environments(P>0.05).The palisade tissue/spongy tissue ratio mirrored the trend of palisade tissue thickness.Light-nitrogen interactions significantly affected palisade tissue thickness of Erythropalum scandens.Light had extremely sig-nificant main effect on palisade tissue/spongy tissue ratio,while both nitrogen main effect and light-nitrogen interactions showed extremely significant effects on palisade tissue/spongy tissue ratio.The results of the fuzzy membership function showed that the average ranking of the membership functions of the 20 light and nitrogen treatment groups as follows:L0N3>L1N2>L0N4>L0N1>L1N4>L0N2>L1N1>L1N3>L1N0>L2N4>L3N4>L0N0>L2N3>L2N2>L3N3>L3N2>L2N0>L2N1>L3N1>L3N0.[Conclusion]The combination of high-intensity light and moderate nitro-gen fertilization(100.00%NS+7.5 g/plant urea)is optimal for leaf growth of Erythropalum scandens cutting seedlings.关键词
赤苍藤/光氮互作/叶片解剖结构/综合评价Key words
Erythropalum scandens/light-nitrogen interaction/leaf anatomical structure/comprehensive evaluation分类
农业科技引用本文复制引用
姜星宇,潘虹,何福英,马道承,王凌晖..光氮互作对赤苍藤苗木叶片解剖结构的影响[J].南方农业学报,2025,56(4):1182-1192,11.基金项目
国家自然科学基金项目(32160359) （32160359）
广西林业科技项目(桂林科研[2021]16号) （桂林科研[2021]16号）
广西研究生教育创新计划资助项目(YCSW2022015) （YCSW2022015）
南宁树木园林下木本蔬菜种植技术研究与示范基金项目(南宁树木园科字2018-1号) National Natural Science Foundation of China(32160359) （南宁树木园科字2018-1号）
Guangxi Forestry Science and Tech-nology Project(Guilinkeyan[2021]16) （Guilinkeyan[2021]16）
Innovation Plan Project of Guangxi Graduate Education(YCSW2022015) （YCSW2022015）
Re-search and Demonstration Fund Project on Planting Technology of Woody Vegetables under Forest of Nanning Arboretum(NNSMYKZ 2018-1) （NNSMYKZ 2018-1）
