南方农业学报2025，Vol.56Issue(4)：1193-1202,10.DOI:10.3969/j.issn.2095-1191.2025.04.017
火龙果SmFT基因克隆及表达分析
Cloning and expression analysis of SmFT gene from pitaya
摘要
Abstract
[Objective]Cloning and spatiotemporal expression pattern analysis of the FT gene(SmFT)of pitaya flower formation were conducted to provide theoretical basis for artificial supplementary light induction of pitaya flower formation and breeding of new light-insensitive pitaya varieties.[Method]The red-fleshed pitaya Jindu 1 was selected as the research object to clone the SmFT gene,which was then performed bioinformatics analysis on.The expression patterns of the SmFT gene in different tissues of pitaya and under the induction of supplementary light at different wave-lengths were detected by real-time fluorescence quantitative PCR.[Result]The open reading frame(ORF)of SmFT gene was 528 bp and encoded 175 amino acid residues.Bioinformatics analysis showed that SmFT protein was an unstable hydrophilic protein with alkalinity,belonging to non-secretory protein and non-membrane protein.Subcellular localiza-tion predicted that SmFT protein was highly likely to be localized in cytoplasm,chloroplast and nucleus.SmFT protein contained a conserved PEBP domain and was closely related to homologous proteins of Vitis vinifera,Vitis serrata and Gypsophila paniculata,while distantly related to homologous proteins of Oxybasis rubra,Chenopodium ficifolium and Amaranthus tricolor.Real-time fluorescence quantitative PCR analysis showed that SmFT gene had obvious tissue expres-sion specificity.The relative expression level of SmFT gene in pitaya calyx was significantly higher than that in other tis-sues,and was as 15.25 times as that in tender floral buds,2.75 times as that in stem tissues adjacent to tender floral buds(P<0.05),and 2.24 times as that in stem buds.The relative expression level of SmFT gene was higher in the stems and stem buds near the tender floral buds of pitaya,while lower in the mature peel,mature flesh,roots and mature stems,and almost not expressed in the mature stems.With the extension of supplementary lighting days,the relative expression of SmFT gene showed a trend of increasing first and then decreasing under the induction of light quality of different wave-lengths,and reached the peak value after 43 d of light induction,and dropped sharply after 50 d(bud stage).The relative expression level of SmFT gene was the highest under the induction of red light with a wavelength of 660 nm,followed by red light of 630 nm and yellow light of 590 nm,and was the lowest under blue light of 450 nm.[Conclusion]SmFT gene has obvious tissue expression specificity and expression differences induced by light of various wavelengths.It is specu-lated that SmFT may be involved in the early floral bud differentiation induction and the later developmental regulation of floral organs or stems,and play an important role in the floral initiation process of pitaya.关键词
火龙果/SmFT基因/基因克隆/表达分析/光周期Key words
pitaya/SmFT gene/gene cloning/expression analysis/photoperiod分类
农业科技引用本文复制引用
武志江,黄黎芳,黄泽键,叶小滢,邓海燕,陆贵锋,梁桂东,韦朔曈,刘朝安,李祯英..火龙果SmFT基因克隆及表达分析[J].南方农业学报,2025,56(4):1193-1202,10.基金项目
国家自然科学基金项目(32202464) （32202464）
广西自然科学基金项目(2023GXNSFBA026055) （2023GXNSFBA026055）
广西农业科学院基本科研业务专项(桂农科2025YP042,桂农科2025YP046) National Natural Science Foundation of China(32202464) （桂农科2025YP042,桂农科2025YP046）
Guangxi Natural Science Foundation(2023GXNSFBA026055) （2023GXNSFBA026055）
Basic Scientific Research Project of Guangxi Academy of Agricultural Sciences(Guinongke 2025YP042,Guinongke 2025YP046) （Guinongke 2025YP042,Guinongke 2025YP046）
