海南医科大学学报2025，Vol.31Issue(9)：659-666,8.DOI:10.13210/j.cnki.jhmu.20240912.003
Rac1/JNK对肺气肿小鼠肺组织细胞凋亡的影响及相关机制研究
Effect of Rac1/JNK on apoptosis of lung tissue cells in mice with emphysema and its related mechanisms
摘要
Abstract
Objective:To investigate the potential mechanism of Rac1/JNK pathway in apoptosis of lung tissue cells in emphy-sema mice.Methods:A total of 40 male C57BL/6 healthy mice were randomly divided into a control group,an emphysema group,an emphysema+Rac1 inhibitor NSC23766 intervention group(emphysema+NSC group),and an emphysema+JNK in-hibitor SP600125 intervention group(emphysema+SP group),with 10 mice in each group.The models were constructed by ciga-rette smoke combined with intraperitoneal injection of cigarette smoke extract for the emphysema+NSC group and the emphyse-ma+SP group.At the beginning of the establishment of the models,each group received intraperitoneal injection of the corre-sponding inhibitor for 28 consecutive days.Western blot was used to detect the expression levels of Rac1,JNK,p-JNK,c-Jun,and Bax proteins in lung tissues.The pathological changes of the lung tissues were observed using H&E staining,the mean lining interval(MLI)and the destruction index(DI)of alveoli were calculated,the relative expression levels of Rac1 mRNA and JNK mRNA in lung tissue were detected by qRT-PCR,the apoptosis of mouse lung tissue cells was evaluated by the TUNEL technol-ogy,and the apoptosis index(AI)was calculated.Result:Compared to the control group,MLI,DI,and AI in the emphysema group were increased(P<0.05),and the expression levels of JNK,Rac1,P-JNK,c-Jun,and Bax and the relative expression levels of Rac1 mRNA and JNK mRNA were increased(P<0.05).Compared to the emphysema group,MLI,DI,and AI in the Rac1 inhibitor and JNK intervention group were decreased(P<0.05),and the expression levels of JNK,Rac1,P-JNK,c-Jun,and Bax and the relative expression levels of Rac1 mRNA and JNK mRNA were decreased(P<0.05).Conclusion:Inhibition of Rac1 can suppress the phosphorylation of JNK in the lung tissue of mice with emphysema and inhibition of the Rac1-JNK pathway can alleviate apoptosis of lung tissue cells in mice with emphysema,which may be related to its reduction in the expression of the pro-apoptotic protein Bax.关键词
Rac1/JNK/肺气肿/细胞凋亡指数Key words
Rac1/JNK/Emphysema/Apoptosis index分类
临床医学引用本文复制引用
金晓迪,罗兰,郑远江,汪洋,李珊珊,钟娟,叶贤伟..Rac1/JNK对肺气肿小鼠肺组织细胞凋亡的影响及相关机制研究[J].海南医科大学学报,2025,31(9):659-666,8.基金项目
This study was supported the Non-profit Central Research Institute Fund of Chinese Academy of Medical Sciences(2019PT320003) 中国医学科学院中央级公益性科研院所基本科研业务费专项资金(2019PT320003) （2019PT320003）
