海南医科大学学报2025，Vol.31Issue(9)：691-701,11.DOI:10.13210/j.cnki.jhmu.20241108.004
基于生物信息学和临床样本探究FMR1与胃癌的相关性及其在肿瘤微环境中的作用
Exploration of the perturbation of FMR1 in gastric cancer and the role in tumor microenvironment based on bioinformatics and clinical samples
摘要
Abstract
Objective:To analyze the expression differences of Fragile X Mental Retardation 1(FMR1)in gastric cancer and adjacent non-tumor tissues,investigate its correlation with the clinical pathological characteristics and outcomes of gastric cancer patients.And to analyze the potential signaling pathways regulated by FMR1 and its role in the tumor microenvironment.Methods:The TIMER2.0 tool was used to analyze FMR1 in the pan-cancer.The Cancer Genome Atlas(TCGA)database was used to ana-lyze differences in the expression of the FMR1 gene between gastric cancer tissues and adjacent normal tissues,and the results were verified by qRT-PCR and immunohistochemistry.The Kaplan-Meier Plottery database was used for survival analysis.R soft-ware and clinical samples were used to assess the correlation between FMR1 expression and clinical pathological factors in gastric cancer patients.The relationship between FMR1 and tumor immune infiltration in gastric cancer was analyzed through the TISIDB database.The function of FMR1 and its interacting genes was analyzed by functional annotation tools.Results:The positive ex-pression rate of FMR1 in gastric cancer was significantly higher than in normal gastric tissue(P<0.001).Patients with high FMR1 expression had a significantly reduced overall survival time(P<0.05).The varying expression of FMR1 in gastric cancer patients was associated with the activity of various immune cells in their tumor microenvironment(P<0.05).FMR1 may be in-volved in processes such as keratin filament and gated channel activities,and was associated with pathways related to olfactory sig-nal transduction,African sleeping sickness,and PPAR signaling.Bioinformatics and clinical sample analysis indicated that the ex-pression level of FMR1 was closely related to the staging of gastric cancer patients(P<0.05).Conclusion:The high expression level of the FMR1 gene is closely associated with poor prognosis and immune cell infiltration in gastric cancer patients,suggesting its potential to be established as a novel biomarker for the diagnosis and treatment of gastric cancer.关键词
胃癌/FMR1/免疫细胞浸润/预后/生物信息学/免疫组化Key words
Gastric cancer/FMR1/Immune cell infiltration/Prognosis/Bioinformatics/Immunohistochemistry分类
临床医学引用本文复制引用
韩宜倩,胡楠,周康杰,蒋薇薇,张淼,毛晨曦,吴风雷..基于生物信息学和临床样本探究FMR1与胃癌的相关性及其在肿瘤微环境中的作用[J].海南医科大学学报,2025,31(9):691-701,11.基金项目
This study was supported by Norman Bethune Medical Science Research Fund Project(KY2023-01-02) （KY2023-01-02）
Lianyungang Health Commission Project(QN202304) （QN202304）
Basic Research Plan Project of Lianyungang Science and Technology Bureau(JCYJ2307) （JCYJ2307）
Key Project of Lianyungang Cancer Prevention and Treatment Technology Development Plan(ZD202302) （ZD202302）
Excellent Talent Fund Project of Xuzhou Medical University Affiliated Hospital Science and Technology Development Fund(XYFY202329) 白求恩医学科学研究基金项目(KY2023-01-02) （XYFY202329）
连云港市卫建委项目(QN202304) （QN202304）
连云港市科技局基础研究计划项目(JCYJ2307) （JCYJ2307）
连云港市肿瘤防治科技发展计划重点项目(ZD202302) （ZD202302）
徐州医科大学附属医院科技发展基金优秀人才基金项目(XY-FY202329) （XY-FY202329）
