华北农学报2025，Vol.40Issue(2)：1-9,9.DOI:10.7668/hbnxb.20195457
小麦转录调控因子Paf1亚基基因的鉴定和逆境响应表达分析
Identification and Stress-responsive Expression Analysis of the Genes for Subunits of Transcription Regulator Paf1 in Wheat
摘要
Abstract
The hexameric Paf1(RNA polymerase Ⅱ associated factor 1)complex is a crucial transcription regulator in eukaryotes.Paf1-regulated expression of specific genes in plants is closely related to diverse biological processes inclu-ding growth,development,and stress responses.In order to get information on the responses of Paf1 to abiotic stresses in common wheat,homologous sequence searches were performed to identify all of the genes encoding each of the Paf1 sub-units in the wheat genome.mCherry fusions of the wheat Paf1 subunit proteins were expressed in protoplasts and tobacco leaves for determination of protein subcellular localization by fluorescence microscopy.qRT-PCR assays were conducted to profile the expression of wheat Paf1 subunit genes in response to different abiotic stresses.The results showed that,in wheat,five of the Paf1 subunits,Ta VIP3,TaVIP4,TaVIP5,Ta VIP6,and TaPHP,were each encoded by one set of homeolo-gous genes while the sixth subunit TaVIP2 was encoded by two sets.Plant VIP2 sequences had an N-terminal proline-rich region with variable length,and wheat TaVIP2 sequences had an additional glutamine-rich region.Protein subcellular lo-calization assays revealed the nuclear localization of TaVIP2,TaVIP4,TaVIP5,and TaVIP6 proteins and the nuclear and cytoplasmic localization of TaVIP3 and TaPHP proteins.Gene expression analyses revealed similar tissue-dependent con-stitutive expression variations and similar stress-induced expression patterns of wheat Paf1 subunit genes.These genes co-ordinately responded to the stress of high temperature by expression upregulation and to the stresses of salt and drought by expression downregulation.Collectively,our results suggested the involvement of expression regulation of Paf1 subunit genes in the responses of wheat to abiotic stresses.关键词
小麦/Paf1复合物亚基/亚细胞定位/胁迫响应Key words
Wheat/Subunit of the Paf1 complex/Subcellular localization/Stress response分类
农业科学引用本文复制引用
崔祎平,李玲珑,陈冬阳,屈展帆,王华忠..小麦转录调控因子Paf1亚基基因的鉴定和逆境响应表达分析[J].华北农学报,2025,40(2):1-9,9.基金项目
国家自然科学基金项目(31971829) （31971829）
天津市高校中青年骨干创新人才培养计划项目(135305JF78) （135305JF78）
天津师范大学中青年教师学术创新推进计划项目(1353P2XC1604) （1353P2XC1604）
