华北农学报2025，Vol.40Issue(2)：10-17,8.DOI:10.7668/hbnxb.20195463
玉米ZmC4NADP-ME基因进化及功能分析
Evolution and Functional Analysis of Maize Gene ZmC4NADP-ME
摘要
Abstract
To explore the function of key genes in photosynthesis,a functional knockout mutant(zmC4nadp-me)of ZmC4NADP-ME,the gene encoding the rate-limiting enzyme of the dark reaction of photosynthesis in maize,was obtained.Evolutionary tree analysis showed that ZmC4NADP-ME and its homologous genes exist in multiple copies in most plants,with diverse expression patterns.Phenotypic analysis revealed that the entire zmC4nadp-me plant was yellow-green,and its seedling-stage leaves dried up and died rapidly under light.Chlorophyll fluorescence analysis indicated that Y(Ⅱ)and electron transport rate ETR(Ⅱ)of photosystem Ⅱ(PS Ⅱ)in zmC4nadp-me decreased significantly,with little change in Y(NPQ),while the Y(NO)increased notably.Measurement of the absorption capacity(P700)of photosystem Ⅰ(PS Ⅰ)found that both the electron transport rate(ETR(Ⅰ))and the actual photoelectron efficiency(Y(Ⅰ))of zmC4nadp-me dropped substantially,and the gap widened with increasing light intensity.Under specific light intensities,Y(ND)and Y(NA)of zmC4nadp-me were greater than those of the wild type(WT).In conclusion,ZmC4NADP-ME is essential for plant growth and development.Disruption of this gene severely stresses PS Ⅱ,and the plant can't alleviate this stress by increasing Y(NPQ).Meanwhile,at low light intensities,the inhibition of PS Ⅰ may originate from the electron donor side of PS Ⅰ,and as the light intensi-ty increases,the inhibition from the electron acceptor side of PS Ⅰ becomes a key factor.关键词
玉米/光合作用/苹果酸酶/光合系统Ⅰ/光合系统ⅡKey words
Maize/Photosynthesis/Malate enzyme/PS Ⅰ/PS Ⅱ分类
农业科学引用本文复制引用
陈华峰,张家宁,张晓,袁月,刘秀峰,刘丹..玉米ZmC4NADP-ME基因进化及功能分析[J].华北农学报,2025,40(2):10-17,8.基金项目
天津市青年基金项目(23JCQNJC00640) （23JCQNJC00640）
河北科技师范学院科学研究基金项目(2024YB020) （2024YB020）
津甘双地科技特派员项目(24CXNL009) （24CXNL009）
2024年度中国博士后科学基金会与天津市联合资助项目(2024T023TJ) （2024T023TJ）
