华北农学报2025，Vol.40Issue(2)：18-26,9.DOI:10.7668/hbnxb.20195345
玉米ZmRAV1基因的克隆分析与功能验证
Cloning,Analysis and Functional Verification of ZmRAV1 Gene
摘要
Abstract
Preliminary transcriptomic analysis identified ZmRAV1 as a candidate gene involved in maize's re-sponse to drought stress.To further investigate its function,this study cloned the ZmRAV1 gene,conducted bioin-formatics analysis of its coding sequence,and overexpressed this gene in Arabidopsis thaliana.The function of Zm-RAV1 was validated by assessing the phenotypes and physiological and biochemical indices of the transgenic Arabi-dopsis lines under drought conditions.The results showed that the ZmRAV1 gene had a total length of 1 176 bp and encoded 389 amino acids.It had the highest proportion of irregular coils in its secondary structure and was a hydro-philic protein that did not contain signal peptides and was non transmembrane.Subcellular localization indicated that the protein was located in the nucleus.ZmRAV1 exhibited high conservation across different species.Phyloge-netic analysis indicated that ZmRAV1 shares the closest evolutionary relationship with its homolog in Miscanthus sinensis,showing a high degree of homology.After drought stress treatment,the root length of Arabidopsis thaliana lines overexpressing ZmRAV1 during germination was significantly higher than that of wild-type(WT)lines.In the seedling stage,WT showed withering or even death after drought stress,while the survival rate was lower than that of overexpressing lines.Moreover,the POD and SOD activities of ZmRAV1 overexpressing lines were higher than those of WT after drought treatment,indicating that overexpression of ZmRAV1 gene could enhance Arabidopsis thaliana's resistance to drought stress.关键词
玉米/干旱胁迫/ZmRAV1/亚细胞定位/功能验证Key words
Zea mays L./Drought treatment/ZmRAV1/Subcellular localization/Functional verification分类
农业科学引用本文复制引用
翟晓婷,王鑫伟,郝喜英,沈俊睿,高旭宏,刘桂雪,刘松涛..玉米ZmRAV1基因的克隆分析与功能验证[J].华北农学报,2025,40(2):18-26,9.基金项目
河北省张家口市科学技术局项目(2311003A) （2311003A）
河北北方学院校级项目(XJ2021016) （XJ2021016）
河北北方学院博士基金项目(BSJJ202225) （BSJJ202225）
