华北农学报2025，Vol.40Issue(2)：27-32,6.DOI:10.7668/hbnxb.20195300
利用CRISPR/Cas9技术编辑SlMYB12基因创制粉果番茄新种质
CRISPR/Cas9-mediated Editing of SlMYB12 for Breeding Pink-fruited Tomato
摘要
Abstract
In order to accelerate the breeding of pink-tomato varieties,a rapid breeding strategy was used to generate pink-fruited tomato material by CRISPR/Cas9-mediated gene editing of SlMYB12.Two adjacent target sites within the first exon of SlMYB12 were selected to construct the CRISPR/Cas9 binary vector,and then the vector was introduced into red-fruited inbred line R18-10C through Agrobacterium-mediated transformation.The homozy-gous mutants without exogenous Cas9 were screened using specific primers and their agronomic traits and fruit nutri-tional quality were analyzed.Sequencing results showed that three homozygous mutants of different mutation types were obtained and all of which were frame shift mutations caused by base deletion.Compared with wild-type red fruit tomatoes,SlMYB12-edited plants grew and developed normally,and there were no significant difference in plant height,single-fruit weight,total yield per plant,fruit total soluble solid content and lycopene level,but the ma-ture fruit showed pink and the flavonoid naringenin chalcone(NarCh)content of tomato peels was significantly re-duced in MYB12-edited plants.In summary,the procedure for the generation of pink-fruited tomato plants through CRISPR/Cas9-mediated targeted mutagenesis of SlMYB12 was set up and new pink-fruited tomato germplasms with stable inheritance were obtained.关键词
番茄/粉果/SlMYB12/CRISPR/Cas9/基因编辑Key words
Tomato/Pink fruit/SlMYB12/CRISPR/Cas9/Gene editing分类
园艺学与植物营养学引用本文复制引用
苏晓梅,吕宏君,刘淑梅,梁增文,陈谦,侯丽霞..利用CRISPR/Cas9技术编辑SlMYB12基因创制粉果番茄新种质[J].华北农学报,2025,40(2):27-32,6.基金项目
山东省农业良种工程项目(2022LZGC009) （2022LZGC009）
中央引导地方科技发展资金项目(YDZX2023028) （YDZX2023028）
山东省科技型中小企业创新能力提升工程项目(2023TSGC0103) （2023TSGC0103）
