华北农学报2025，Vol.40Issue(2)：33-39,7.DOI:10.7668/hbnxb.20195260
马铃薯StPHR1基因克隆与表达特征分析
Cloning and Expression Characteristic Analysis of StPHR1 from Potato
摘要
Abstract
PHR1 is a crucial factor in balancing plant disease resistance and low phosphorus stress resistance.To investigate the nature and function of the StPHR1 gene in potato and to explore the role of StPHR1 in the process of potato resistance to Alternaria solani infection,the CDS sequence of the StPHR1 gene was cloned by PCR technol-ogy using potatoes as the research material,and the structural,physicochemical properties,and phylogenetic relation-ships of StPHR1 were analyzed and predicted using bioinformatics software,then,the expression level of StPHR1 during the infection of potatoes by A.solani and under different hormone treatments was analyzed using qRT-PCR technology,and subcellular localization analysis of the protein was conducted using laser confocal microscopy tech-nology.The results showed that the CDS of the StPHR1 gene was 1 353 bp,encoding 450 amino acids.The protein had a molecular formula of C2147H3399N595O711S18,a molecular weight of 49.51 ku,and a theoretical isoelectric point of 5.07,encoding a hydrophilic,unstable protein with no signal peptide and no transmembrane structure.Its second-ary structure consisted mainly of random coil and α-helix.Phylogenetic tree analysis revealed that the StPHR1 pro-tein was most closely related to Arabidopsis thaliana;conservative domain analysis revealed that the StPHR1 pro-tein,like other PHR1s,possesses both MYB-CC and MYB conserved structural domains at its C-terminus.Relative expression analysis found that StPHR1 was significantly induced by A.solani and salicylic acid,and it was hypothe-sized that StPHR1 played an important role in A.solani infection of potato and in the response to salicylic acid;and the subcellular localization showed that the StPHR1 protein was localized in the nucleus.It is hypothesized that StPHR1 may regulate potato resistance to A.solani through its MYB transcription factor activity and response to sali-cylic acid.关键词
马铃薯/早疫病菌/StPHR1/生物信息学分析/亚细胞定位Key words
Potato/Alternaria solani/StPHR1/Bioinformatics analysis/Subcellular localization分类
农业科学引用本文复制引用
彭泽驰,姜海滨,丁丽丽,杨志浩,杨志辉,朱杰华..马铃薯StPHR1基因克隆与表达特征分析[J].华北农学报,2025,40(2):33-39,7.基金项目
现代农业产业技术体系建设专项资金(CARS-09-P18) （CARS-09-P18）
河北省薯类产业技术体系创新团队专项基金项目(HBCT2023060205) （HBCT2023060205）
