华北农学报2025，Vol.40Issue(2)：40-48,9.DOI:10.7668/hbnxb.20195485
西葫芦种子无壳性状遗传分析及无壳基因Cphl初步定位
Genetic Analysis and Preliminary Mapping of Seed Hull-less Gene Cphl in Zucchini
摘要
Abstract
The naked seeds of zucchinii possess significant natural advantages in processing.To investigate the genetic mechanisms underlying the hull-less trait in zucchini,we utilized the hulled 17pu10(P1)and the hull-less 17pu08(P2)as parental strains,and constructed populations of F1(P1×P2),F2,and BC1.The phenotypes of the zucchini seeds in the progeny population were assessed.The results showed that the number ratio of hulled seeds and hull-less seeds in the progeny population was in line with 3∶1 separation ratio.This indicated that the hull-less trait was regulated by a single gene and that the hull-less allele was recessive.Genetic mapping within this interval revealed that the gene for hull-less in zucchini was located between the markers InDel3157329 and InDel3724121,with genetic distances of 1.4,2.6 cM,respectively,while the physical distance was 0.6 Mb.The annotation and function analysis of 24 genes in the interval showed that 4 genes were directly or indirectly involved in the biosyn-thesis of cell wall,cellulose and lignin.Further analysis of the expression differences of 4 genes showed that only Cp4.1 LG12g04350 and Cp4.1 LG12g04370 had significant differences in expression levels during seed develop-ment.It was inferred that Cp4.1LG12g04350 or Cp4.1LG12g04370 was the candidate gene controlling the hull-less trait.In addition,InDel markers linked to hull-less genes were developed,which could be used as markers to identify zucchini hull-less traits,so as to accelerate the breeding of high-quality zucchini seed hull-less varieties.关键词
西葫芦/无壳基因/遗传分析/基因定位/基因连锁/InDel标记Key words
Zucchini/Hull-less gene/Genetic analysis/Gene mapping/Gene linkage/InDel marked分类
园艺学与植物营养学引用本文复制引用
董晨晨,刘泽慧,曹嫒婉,许小勇,雷逢进,刘庆华..西葫芦种子无壳性状遗传分析及无壳基因Cphl初步定位[J].华北农学报,2025,40(2):40-48,9.基金项目
山西省自然科学研究面上项目(202103021224125) （202103021224125）
国家重要特色物种西葫芦育种联合攻关子项目(NYGG20-2) （NYGG20-2）
