华北农学报2025，Vol.40Issue(2)：49-55,7.DOI:10.7668/hbnxb.20195402
烟草NtPsbS的亚细胞定位及编码基因表达模式分析
Subcellular Localization and Coding Gene Expression Pattern Analysis of NtPsbS in Tobacco
摘要
Abstract
In order to reveal the expression patterns of PsbS protein in Nicotiana tabacum,the full-length NtPs-bS gene sequence was cloned from cDNA of the tobacco cultivar K326,and the multiple protein sequences of NtPsbS gene and PsbS genes of seven crops including rice,tomato and soybean were compared by DNAMAN software.The phylogenetic analysis was carried out by MEGA 11 software,and the phylogenetic tree was established by neighbor-joining method.Tissue expression of NtPsbS gene in tobacco at different growth stages was detected by qRT-PCR.The plant expression vector pS1300-PsbS-GFP was constructed to find subcellular localization of the NtPsbS mature protein.Finally,the mRNA levels of NtPsbS under abiotic stresses were analyzed in K326.The results showed that the total length of tobacco NtPsbS gene was 825 bp,encoding 274 amino acids.The homology of tobacco NtPsbS pro-tein and tomato SlPsbS protein was the highest up to 91％.The NtPsbS gene exhibited the highest mRNA levels in the leaves among the tissues,including leaves,roots,stems,seeds and other parts of K326.The mature NtPsbS pro-tein was located in chloroplasts.For the abiotic stress,it was found that the expression level of NtPsbS was signifi-cantly increased under salt stress,cold stress and Abscisic acid(ABA)treatments.In summary,the expression level of NtPsbS was the highest in tobacco leaves at different growth stages,and it was higher when under the treatments of salt stress,cold stress and ABA,indicating that this gene may be involved in the salt and cold stress resistance and ABA metabolic pathway in tobacco,which could provide basis and reference for the functional analysis of the NtPsbS gene in future.关键词
普通烟草/PsbS基因/亚细胞定位/组织表达模式/非生物胁迫Key words
Nicotiana tabacum/PsbS gene/Subcellular localization/Tissue expression pattern/Abiotic stress分类
农业科学引用本文复制引用
范普晴,周厚良,宋杉杉,林法明,石永春,王潇然,王燃,张小全..烟草NtPsbS的亚细胞定位及编码基因表达模式分析[J].华北农学报,2025,40(2):49-55,7.基金项目
国家烟草专卖局科技项目(110202101042 （）
110202102033 （）
110202202038) （）
河南农业大学高层次人才支持项目(30501301) （30501301）
