华北农学报2025，Vol.40Issue(2)：81-91,11.DOI:10.7668/hbnxb.20195230
野生稻SSSLs孕穗期对干旱胁迫的响应
Response to Drought Stress of Wild Rice SSSLs at Booting Stage
摘要
Abstract
To reveal the mechanism of drought resistance rice in booting stage,six single chromosome segments substitution lines(SSSLs)constructed from Oryza meridionalis and O.glumaepatula and their recipient parent Hua-jingxian 74(HJX74)were used as experimental materials for potted drought treatment.Six biochemical indexes during 0,5,10 days of drought treatment and 5 days of rewatering and 11 agronomic traits after setting stage were measured,and the drought tolerance of 7 materials was comprehensivly evaluated by correlation analysis and princi-pal component analysis.The result showed that under drought stress,there were extremely significant difference in agronomic traits among the 7 materials.There were extremely significant difference between M78-1 and HJX74 in relative panicle length,relative empty grain number and relative grain number per panicle,and there was extremely significant difference between M148 and HJX74 in relative dented grain number,there were extremely significant differences between M107 and HJX74 in relative panicle length and relative number of secondary branches;six drought-tolerant QTLs were identified at booting stage,including qRPL1-1,qRPL2-1,qRNSB2-1,qRNDG11-1,qRNEG1-1,qRGNP1-1,which were distributed on chromosomes 1,2,and 11.The superoxide dismutase(SOD)ac-tivity and peroxidase(POD)activity increased by 3.76％—18.20％and 31.88％—100.00％after 5 days of drought,while malondialdehyde(MDA)concentration decreased by 41.07％—81.65％.After 10 days of drought,SOD activity and POD activity decreased by 9.20％-48.53％and 44.74％—79.79％,while malondialdehyde(MDA)concentration was extremely significantly higher than that on the 5 th day of drought.Osmoregulatory sub-stances such as proline,soluble sugar and soluble protein continued to increase at the 5 d and 10 d drought treat-ment stages,and the biochemical indexes basically returned to normal level after 5 days of rewater.The comprehen-sive analysis revealed that the eigenvector and contribution rates of relative seed setting rate,POD activity and pro-line were the largest,indicating that these three indexes could better represent the drought tolerance of rice at boo-ting stage.In conclusion,drought stress can affect agronomic traits and biochemical indexes of rice at booting stage,and rice can regulate its metabolic process in response to drought stress.关键词
水稻/孕穗期/干旱胁迫/农艺性状/生化指标/耐旱综合评价Key words
Rice/Booting stage/Drought stress/Agronomic trait/Biochemical index/Comprehensive evalu-ation of drought tolerance分类
农业科技引用本文复制引用
陈科,廖冰,刘国利,黄秀艳,何平..野生稻SSSLs孕穗期对干旱胁迫的响应[J].华北农学报,2025,40(2):81-91,11.基金项目
国家自然科学基金面上项目(32171967) （32171967）
广东省自然科学基金自由申请项目(2019A1515010820) （2019A1515010820）
广州市科技计划项目(201904010183) （201904010183）
