华北农学报2025，Vol.40Issue(2)：153-160,8.DOI:10.7668/hbnxb.20195359
基于BSA-seq和RNA-seq技术挖掘黄瓜白粉病抗性基因
Gene Mining of Resistance to Powdery Mildew Based on BSA-seq and RNA-seq in Cucumber
摘要
Abstract
Cucumber powdery mildew is one of the main diseases that adversely impacts cucumber production,posing a significant challenge to its sustainable cultivation.Identifying genes related to cucumber resistance to pow-dery mildew can help understand the genetic principles and molecular mechanisms of cucumber resistance to pow-dery mildew,and provide diverse gene resources for disease resistant breeding.This study constructed F1 and F2 populations of QK×QG using cucumber resistant inbred line QK and susceptible inbred line QG as parents.Using the extreme trait mixed pool resequencing(BSA-seq)method,the genomic regions harboring the resistance genes of cucumber powdery mildew were preliminarily located.By integrating transcriptome data with gene annotation infor-mation,the association interval of the disease phenotype was narrowed,sequence variations were identified,and key genes were screened.The results showed that the resistance of powdery mildew may be controlled by recessive genes,and the population of F2 showed a continuous normal distribution from resistant to susceptible.The BSA-seq analysis,combining the SNP-Index method and QTG(quantitative trait genomics)-seq method analysis highlighted the 19-21 Mb region of chromosome 5,where there were 77 annotated genes with SNP differences between sam-ples,including 33 non-synonymous mutations.The transcriptome sequencing(RNA-seq)results showed that there were 309 upregulated genes and 697 downregulated genes in the susceptible material.The expression levels of 13 genes within the candidate segment of chromosome 5 showed significant differences after infection.Through a com-prehensive analysis of differentially expressed genes and BSA,the candidate genes in this segment were narrowed to 3,and only SNP mutations were detected in the LOC101207011 gene.The candidate gene LOC101207011 was char-acterized by a mutation resulting in an amino acid change from Valine at position 656 to Leucine.This gene emerges as the primary focus of our investigation due to its potential role in conferring resistance to powdery mildew.关键词
黄瓜/BSA-seq/RNA-seq/白粉病/定位Key words
Cucumber/BSA-seq/RNA-seq/Powdery mildew/Mapping分类
农业科技引用本文复制引用
秦垒,张巧英,孙雷,石昊颖,檀海斌,石学萍..基于BSA-seq和RNA-seq技术挖掘黄瓜白粉病抗性基因[J].华北农学报,2025,40(2):153-160,8.基金项目
河北省重点研发计划项目(21326342D) （21326342D）
