化工进展2025，Vol.44Issue(5)：2441-2450,10.DOI:10.16085/j.issn.1000-6613.2024-1843
毕赤酵母利用甲醇生产重组蛋白技术的研究进展
Research progress on bioproduction of recombinant proteins by Pichia pastoris utilizing methanol
摘要
Abstract
As green and sustainable low-carbon feedstocks without competition with people for food,methanol and other C1 compounds have great potential for advancement of biomanufacturing.Pichia pastoris(P.pastoris),as an industrial strain capable of naturally utilizing methanol,has a long history of research and has been widely employed in the biomanufacturing of various recombinant proteins.It has become an excellent chassis cell factory for methanol utilization.In recent years,there has been progress in understanding the microbial physiology,methanol metabolism networks,protein secretion expression pathways,and the identification of key components in P.pastoris.However,several critical challenges remain that limit its efficient application,such as limited understanding of the complex mechanisms of cell toxicity caused by methanol and its metabolites,insufficient capacity to explore"biological dark matters"related to cell robustness and methanol utilization,and restricted engineering approaches for efficiently converting methanol into target proteins.This review summarizes the research progress on the bioconversion of methanol into recombinant proteins by P.pastoris,with the emphasis on discussing how to optimize the methanol conversion and protein expression in P.pastoris cell factory.Finally,we address the challenges encountered in the recombinant protein production from methanol in P.pastoris and provide an outlook on its future biotechnology innovation and potential application.关键词
甲醇/毕赤酵母/重组蛋白/代谢工程/合成生物学Key words
methanol/Pichia pastoris/recombinant proteins/metabolic engineering/synthetic biology分类
化学工程引用本文复制引用
王媛媛,张翀,韩双艳,邢新会..毕赤酵母利用甲醇生产重组蛋白技术的研究进展[J].化工进展,2025,44(5):2441-2450,10.基金项目
国家重点研发计划(2018YFA0901500) （2018YFA0901500）
深圳市高等院校稳定支持计划(WDZC20231126172733002). （WDZC20231126172733002）
