化工进展2025，Vol.44Issue(5)：2489-2504,16.DOI:10.16085/j.issn.1000-6613.2024-1598
微生物细胞活力调控生产丁二酸
Regulation of microbial cell viability for succinic acid production
摘要
Abstract
With the development of metabolic engineering technologies,microbial fermentation for the production of succinic acid has received widespread attention.Currently,various engineered strains for high succinic acid production have been developed,including Escherichia coli,Mannheimia succiniciproducens,Actinobacillus succinogenes,Corynebacterium glutamicum,and Yarrowia lipolytica.However,due to the lack of microbial cell viability in the later stages of succinic acid fermentation,production efficiency significantly decreases,thus limiting the efficient production and industrial application of succinic acid.This article discusses strategies and methods for enhancing microbial cell viability in succinic acid microbial fermentation,focusing on chemical engineering methods based on the supply of specific internal and external compounds,metabolic engineering strategies to improve cell growth performance and environmental adaptability,and fermentation process optimization.Finally,the article provides an outlook on the industrial application of microbial fermentation for succinic acid production.关键词
丁二酸/大肠杆菌/细胞活力/代谢工程/生物制造Key words
succinic acid/Escherichia coli/cell viability/metabolic engineering/biomanufacturing分类
生物工程引用本文复制引用
唐永圣,顾子蕴,陈修来..微生物细胞活力调控生产丁二酸[J].化工进展,2025,44(5):2489-2504,16.基金项目
国家重点研发计划(2019YFA0904900) （2019YFA0904900）
中央高校基本科研业务费专项资金(JUSRP622001). （JUSRP622001）
