环境工程学报2025，Vol.19Issue(4)：772-783,12.DOI:10.12030/j.cjee.202401057
耐寒抗逆藻种筛选及其处理寒区沼液效果
Screening of low temperature and stress-resistant microalgae species and their effect on cold areas biogas slurry treatment
摘要
Abstract
In addressing challenges related to high energy consumption and low efficiency in utilizing microalgae for biogas slurry resource utilization under cold conditions,six microalgae strains adaptable to 15℃ were screened.Their tolerance to NH4+-N and pollutant removal from biogas slurry were investigated.The release mechanisms of the low-temperature inhibition were also explored.At 15 ℃,the six strains could tolerant 400 mg·L-1 NH4+-N,and the biomass concentration reached 204.67～223.79 mg·L-1.Chlorophyll a,chlorophyll b,and carotenoid concentrations ranged from 4.77～5.84,1.73～2.23,and 1.46～2.09 mg·L-1,respectively.NH4+-N and TP removal ranged from 44.09％～47.02％and 6.63％～15.60％,respectively.After 10 days'cultivation,Chlamydomonas(W-4)exhibited the highest biomass(223.79 mg·L-1)and NH4+-N(47.02％)and TP(15.60％)removal.The total chlorophyll,chlorophyll a,chlorophyll b,and carotenoids concentration were 8.07,5.84,2.23,and 2.09 mg·L-1,respectively,which increased by 17.09％～55.81％,7.66％～9.49％,264.49％～437.93％,35.89％～42.88％,46.97％～47.87％,13.48％～31.27％,and 31.21％～42.73％compared with polar Stichococcus antarticus and common Chlamydomonas,respectively.The enhanced carotenoid content in the screened microalgae suggested that the mitigation of the oxidative stress(ROS)damage in microalgae cells at low temperatures by the high nutrient levels might be the main reason for promoting the microalgal biomass concentration.Results revealed that the screened strains exhibited high tolerance to the low-temperature and high NH4+-N concentration.The growth period was shorter than the other previous species,which providing valuable genetic resources for cold region in microalgae cultivation and biogas slurry utilization.关键词
低温微藻/寒区种质资源/猪粪沼液/耐寒抗逆性Key words
low-temperature microalgae/germplasm resources in cold regions/pig manure biogas slurry/cold and stress resistance分类
环境科学引用本文复制引用
王翠翠,卢海凤,刘德钊,张光明,司哺春,蒋伟忠,李保明..耐寒抗逆藻种筛选及其处理寒区沼液效果[J].环境工程学报,2025,19(4):772-783,12.基金项目
国家自然科学基金区域创新发展联合基金项目(U21A20162) （U21A20162）
