黑龙江农业科学Issue(5)：83-89,7.DOI:10.11942/j.issn1002-2767.2025.05.0083
苏打盐碱胁迫对辐射诱变后苜蓿组培苗生理特性的影响及其抗性综合评价
Effects of Soda Salt Alkali Stress on Physiological Characteristics of Aalfalfa Tissue Culture Seedlings After Radiation Mutagenesis and Comprehensive Evaluation of Their Resistance
摘要
Abstract
In order to screen soda salt alkali resistant alfalfa germplasm materials,the experiment used three types of tissue culture seedlings as materials,subjected them to soda salt alkali stress,and analyzed the physiological response of the three tissue culture seedlings to stress.The results showed that different physiological responses of irradiated and non irradiated alfalfa tissue cultured seedlings under soda salt stress,resulting in different changes in soluble protein(SP)conten,proline(Pro)conten,soluble sugar(SS)content,POD activity,CAT activity,SOD activity,MDA content,and relative conductivity(RC).Under the stress of 150 mmol·L-1 soda salt alkali(NaHCO3∶Na2CO3=9∶1),the various indicators of G3 and G4 were compared.The SP(7.41 mg·g-1),SS(3.38％ ),Pro(54.60 μg·g-1),POD(87 277.78 U·min-1·g-1),and CAT(2 746.67 U·min-1·g-1)of G3 were higher than those of G4,while SOD(8.05 U·min-1·g-1),MDA(129.85 μmol·g-1),and RC(42.07％ )were lower than those of G4.The sensitivity of each individual indicator to soda salt alkali stress varies,with soluble protein and peroxidase being relatively more sensitive.Based on the comprehensive analysis of the membership function values of the resistance coefficients of 8 physiological indicators to soda salt alkali stress,the resistance ability of three alfalfa tissue culture seedlings to soda salt alkali was G3＞G4＞CK.60Co-γ radiation can enhance the salt alkali resistance of alfalfa tissue culture seedlings,and the research results can provide reference for the breeding of alfalfa salt alkali resistant mutants using radiation technology.关键词
苜蓿组培苗/60Co-γ辐射/苏打盐碱胁迫/生理特性/综合评价Key words
alfalfa tissue culture seedling/60Co-γ radiation/soda salt alkali stress/physiological characteristics/comprehensive evaluation引用本文复制引用
陈雪梅,刘畅,朱琨,李波..苏打盐碱胁迫对辐射诱变后苜蓿组培苗生理特性的影响及其抗性综合评价[J].黑龙江农业科学,2025,(5):83-89,7.基金项目
黑龙江省省属高等学校基本科研业务费科研项目(145109312,135409216) （145109312,135409216）
2021年大学生创新创业训练计划项目(国家级重点领域,202110232012) （国家级重点领域,202110232012）
2023 年国家级大学生创新创业训练计划项目(202310232055). （202310232055）
