河南农业科学2025，Vol.54Issue(4)：47-56,10.DOI:10.15933/j.cnki.1004-3268.2025.04.005
烟草NtHPL基因特征及其在TMV胁迫下的表达分析
Characterisation of the NtHPL Genes and Their Expression Pattern under TMV Stress in Tobacco
摘要
Abstract
Hydroperoxide lyase(HPL)is a key enzyme in the catalytic fatty acid oxidation pathway and belongs to the cytochrome P450(CytP450)superfamily.HPL plays an essential role in plant responses to both biotic and abiotic stresses,contributing significantly to the regulation of stress tolerance.In this study,the NtHPL gene family in tobacco was identified using genomic data and comprehensively analyzed for their physicochemical properties,protein structures,conserved motifs,and response patterns to tobacco mosaic virus(TMV).The results indicated that nine NtHPL family members were identified,distributed across eight chromosomes,and classified into three subclasses:B,C,and D.The amino acid lengths of these members ranged from 437 to 527 residues,with theoretical isoelectric points(pI)varying between 5.84 and 9.15.Subcellular localization predictions suggested that these proteins were primarily localized in chloroplasts and cytoplasm.Expression analysis revealed that upon TMV infection,five NtHPL genes were upregulated,one was downregulated,and three exhibited no significant differential expression in tobacco leaves.Overexpression of NtHPL1 enhanced tobacco resistance to TMV,as demonstrated by experimental evidence.These findings collectively suggest that NtHPL may be involved in the regulatory mechanisms underlying tobacco defense against TMV.关键词
烟草/脂氢过氧化物裂解酶/烟草花叶病毒/细胞色素P450Key words
Tobacco/Hydroperoxide lyase/TMV/Cytochrome P450分类
农业科技引用本文复制引用
王明鑫,刘磊,邓兆龙,王昊阳,刘鹏飞,金维环,李俊营,郭红祥..烟草NtHPL基因特征及其在TMV胁迫下的表达分析[J].河南农业科学,2025,54(4):47-56,10.基金项目
平顶山市烟草公司科技项目(PYKJ202206) （PYKJ202206）
