河南农业科学2025，Vol.54Issue(4)：57-65,9.DOI:10.15933/j.cnki.1004-3268.2025.04.006
基于转录组的芍药WOX基因家族鉴定及PlWOX5在盐胁迫下的功能分析
Identification of the WOX Gene Family in Paeonia lactiflora Based on Transcriptome Data and Functional Analysis of PlWOX5 Under Salt Stress
摘要
Abstract
In order to clarify the function,evolutionary relationship,and role in biological processes of the Paeonia lactiflora WOX gene family,members of Paeonia lactiflora WOX family(PlWOXs)were identified based on transcriptome data,followed by a comprehensive bioinformatics analysis of their protein physicochemical properties,structures,evolutionary relationships,and expression patterns.The results showed that five PlWOX genes were identified based on the transcriptome data of Paeonia lactiflora,and their encoded amino acids were mainly hydrophobic and unstable alkaline nucleoproteins.The results of the system evolution analysis showed that the PlWOXs gene was distributed in both WUS/modern lineages and ancient lineages,with no PlWOXs gene distribution in the intermediate lineages.The results of protein structure analysis indicated that there were differences in the distribution and quantity of Motif on different PlWOXs genes,and they had significant tissue specificity.PlWOX2 and PlWOX5 were mainly highly expressed in Paeonia lactiflora leaves.The qRT-PCR detection results showed that PlWOXs genes were responsive to salt stress,but PlWOX5 showed a more significant response to salt stress.Overexpression of PlWOX5 gene could enhance salt tolerance in Arabidopsis by enhancing antioxidant enzyme activity(superoxide dismutase,peroxidase,catalase)and increasing the content of osmoregulatory substances(soluble proteins)to promote reactive oxygen species(ROS)clearance.In conclusion,PlWOX5 gene can improve the salt tolerance of Paeonia lactiflora by increasing the activity of antioxidant enzymes and the content of osmotic regulators.关键词
WOX基因家族/芍药/盐胁迫/抗氧化酶活性/渗透调节物质Key words
WOX gene family/Paeonia lactiflora/Salt stress/Antioxidant enzyme activity/Osmotic regulatory substance分类
农业科技引用本文复制引用
彭波,马梦梅,赵苹..基于转录组的芍药WOX基因家族鉴定及PlWOX5在盐胁迫下的功能分析[J].河南农业科学,2025,54(4):57-65,9.基金项目
国家自然科学基金项目(31970118) （31970118）
