河南农业科学2025，Vol.54Issue(4)：80-90,11.DOI:10.15933/j.cnki.1004-3268.2025.04.008
淹水胁迫对香根草根际土壤氮转化及其细菌群落结构多样性的影响
Effects of Submerged Stress on Inter-root Soil Nitrogen Transformation of Vetiveria zizanoides and Structural Diversity of Bacterial Communities
摘要
Abstract
In order to study the effect of inter-root bacterial community diversity on soil nitrogen transformation in the vegetation of Danjiangkou Reservoir fallout zone,Vetiveria zizanoides was selected as experimental material,and control(CK),semi-submerged and fully submerged groups were set up to systematically study the effect of submerged stress on inter-root nitrogen transformation and bacterial community through soil physicochemical analyses,enzyme activity determination combined with high-throughput sequencing of 16S rRNA and function prediction of PICRUSt2.The results showed that the content of inter-root soil ammonium nitrogen(NH4+-N)was significantly elevated under submerged stress,and the content of nitrate nitrogen(NO3--N)and nitrite nitrogen(NO2--N)were significantly reduced by 66.83%—74.27%and 36.84%—42.11%,respectively,compared with CK.Compared with CK,soil hydroxylamine reductase(HR)activity in the fully submerged group was significantly reduced by 34.48%,and nitrate reductase(NR)and nitrite reductase(NiR)activities were significantly increased.The results of Alpha diversity analysis showed that flooding stress significantly increased the diversity of inter-root bacterial communities,and the order was fully submerged group>semi-submerged group>CK.The non-metric multidimensional scaling analysis and principal component analysis showed that the bacterial community structure showed gradient differences with the increase of submerged depth.Community structure analysis showed that the relative abundance of Firmicutes in the semi-submerged and fully submerged groups increased significantly by 45.14%and 87.99%,respectively,while the relative abundance of Cyanobacteria in the fully submerged group decreased significantly by 84.98%.Functional prediction revealed that the abundance of genes for the key enzymes of biological nitrogen fixation pathway(Nitrogenase 1.18.6.1)was significantly increased by 198.43%to 198.97%under submerged stress,but it suppressed the abundance of genes for nitrous oxide reductase(1.7.2.4).Redundancy analysis indicated that soil NO3--N and NR activities were the main factors affecting the soil bacterial community.In conclusion,submerged stress affects the nitrogen transformation process in inter-root soils and alters bacterial community structure,which may lead to ecological risks of N2O greenhouse gas accumulation.关键词
香根草/根际细菌群落/淹水胁迫/氮转化/功能预测/丹江口水库消落带Key words
Vetiveria zizanoides/Inter-root bacterial community/Submerged stress/Nitrogen transformation/Functional prediction/Danjiangkou Reservoir fallout zone分类
农业科技引用本文复制引用
魏家婷,胡田,赵睿博,刘影,姬明飞,张君..淹水胁迫对香根草根际土壤氮转化及其细菌群落结构多样性的影响[J].河南农业科学,2025,54(4):80-90,11.基金项目
河南省科技攻关项目(242102321168) （242102321168）
南阳师范学院青年基金培育项目(2023PY021) （2023PY021）
