河南农业科学2025，Vol.54Issue(4)：101-108,8.DOI:10.15933/j.cnki.1004-3268.2025.04.010
绿豆对咪唑啉酮除草剂的抗药性评价及交互抗性分析
Resistance Evaluation and Cross-resistance Analysis of Mung Bean to Imidazolinone Herbicides
摘要
Abstract
To precisely determine the resistance levels of Vigna radiata to imazethapyr and imazapic,explore the underlying molecular mechanisms,and assess potential cross-resistance and multi-resistance to other herbicides,the resistance levels of 27 V.radiata varieties to imazethapyr and imazapic were measured using the agar solid medium method.Additionally,the sensitive and resistant varieties were selected to evaluate their cross-resistance and multi-resistance to other herbicides.Finally,the mechanism through which V.radiata developed resistance to imidazolinone herbicides was elucidated via acetolactate synthase gene amplification and sequence alignment analysis.The results indicated that the preliminary screening results of resistance of 27 V.radiata varieties to imazethapyr revealed that seven varieties were sensitive,while five varieties were resistant,and one variety was suspected resistant.Both imazethapyr and imazapic could inhibit the growth and development of V.radiata.The resistance results of 13 V.radiata varieties to imazethapyr showed that seven varieties were identified as sensitive,accounting for 53.85%of the total,while six varieties exhibited low-level resistance,accounting for 46.15%.Specifically,Jilü H1004 was the most sensitive variety,with a GR50 value of 18.80 mg/kg.Baolü 2016 22-2 demonstrated the highest resistance,having a resistance index(RI)of 3.63.The resistance results of 13 V.radiata varieties to imazapic showed that seven varieties(53.85%)showed high resistance and five(38.46%)displayed extremely high resistance.Pinlü 2019-26-8-17 had the highest resistance level,with a RI value of 237.85.The findings from the ALS gene sequence analysis indicated that there were no disparities in the amino acid sequences of the two ALS genes between the relatively resistant and relatively sensitive V.radiata varieties.This suggested that the variation in the resistance of V.radiata to imidazolinone herbicides was not attributable to mutations at the ALS gene site.The results of the cross-resistance analysis revealed that the relatively resistant cultivar Pinlü 2019-26-8-17 exhibited cross-resistance to choransulam-methyl and halosulfuron-methyl,with the RI values of 500.71 and 170.47,respectively.The results of the multi-resistance analysis indicated that this relatively resistant cultivar demonstrated multi-resistance to fluroxypyr,with a RI value of 4.38.However,it did not show multi-resistance to bentazone,bixlozone and lactofen.In conclusion,among the tested varieties,there were variations in the resistance levels of different V.radiata varieties to imidazolinone herbicides.Moreover,the relative resistant cultivar exhibited multi-resistance to fluroxypyr.These findings provide a theoretical reference for effectively controlling weeds in V.radiata fields.关键词
绿豆/抗除草剂/咪唑乙烟酸/甲咪唑烟酸/交互抗性/多抗性Key words
Vigna radiata/Resistance to herbicides/Imazethapyr/Imazapic/Cross-resistance/Multi-resistance分类
农业科技引用本文复制引用
段云,苏旺苍,薛飞,王春雨,徐洪乐,孙兰兰,吴仁海..绿豆对咪唑啉酮除草剂的抗药性评价及交互抗性分析[J].河南农业科学,2025,54(4):101-108,8.基金项目
河南省科技研发计划联合基金(优势学科培育类)项目(232301420113) （优势学科培育类）
河南省农业科学院科技创新团队项目(2022TD08) （2022TD08）
