湖南中医药大学学报2025，Vol.45Issue(4)：588-597,10.DOI:10.3969/j.issn.1674-070X.2025.04.002
清心宁神汤对心不藏神不寐模型大鼠的药效实验研究
Pharmacodynamic study of Qingxin Ningshen Decoction on a rat model of insomnia due to heart not storing spirit
摘要
Abstract
Objective To explore the therapeutic effects of Qingxin Ningshen Decoction(QXNSD)on a rat model with insomnia due to heart not storing spirit,induced by caffeine combined with 4-chloro-DL-phenylalanine(PCPA).Methods Thirty-six SD rats were randomly divided into a normal group,a model group,a zolpidem group,low-dose and high-dose QXNSD groups,and a pathway inhibitor group,with six rats in each group.The rat model with insomnia due to heart not storing spirit was established using caffeine and PCPA.The normal group received an equal volume of saline via intraperitoneal injection once daily for 10 consecutive days;the remaining groups received an intraperitoneal injection of 60 mg/kg caffeine solution from days 1 to 7,followed by a subcutaneous injection of 300 mg/kg PCPA from days 8 to 10.After modeling,the zolpidem group was administered 1.05 mg/(kg·d)zolpidem tartrate solution by gavage;the low-and high-dose QXNSD groups were given 14.7 and 29.4 g/(kg·d)QXNSD by gavage;and the pathway inhibitor group was given 0.3 mg/(kg·d)phosphatidylinositol kinase inhibitor by intraperitoneal injection.The normal and the model groups were gavaged with an equal volume of normal saline.All interventions were administered for 14 consecutive days.After the intervention,the open field test was conducted to assess the spontaneous activity and anxiety level of the rats,evaluating the ameliorative effects of QXNSD on sleep and anxiety disorders of rats with insomnia due to heart not storing spirit.ELISA was used to measure the levels of interleukin-1β(IL-1β)and C-reactive protein(CRP)in the serum of rats;the immunohistochemical staining was applied to assess the IL-1β levels.HE staining,Nissl staining,and Luxol fast blue staining were employed to observe pathological changes in brain tissue.Results Compared with the normal group,the model group showed significant reductions in total sleep time(TST),slow-wave sleep(SWS)time,rapid eye movement(REM)sleep time,total movement distance,total activity time,central activity time,and rearing time(P＜0.01).Meanwhile,fecal pellet count,serum IL-1β and CRP levels,and IL-1β expression in brain tissue were significantly higher(P＜0.01).Neurological tissue exhibited a certain degree of structural disorganization with inflammatory cell infiltration.Compared with the model group,both the low-and high-dose QXNSD groups showed significant increases in TST,SWS time,REM time,total movement distance,total activity time,central activity time,and rearing time(P＜0.01),while fecal pellet count,serum IL-1β and CRP levels,and IL-1β expression in brain tissue were significantly lower(P＜0.01).The results of HE staining,Nissl staining,and Luxol fast blue staining indicated that in both the low-and high-dose QXNSD groups,neuronal arrangement was largely intact,with basically normal nuclear size,shape,and position.Mild hyperchromatic nuclei and limited glial cell proliferation were observed,and central Nissl body dissolution was alleviated compared to the model group.No significant demyelination was noted,and lighter-stained areas showed varying degrees of improvement.Conclusion QXNSD exhibits significant therapeutic effects on rats with insomnia due to heart not storing spirit.It can effectively alleviate insomnia and anxiety disorders,reduce the levels of relevant inflammatory factors in the serum,and improve pathological changes in neuronal cells caused by insomnia.关键词
清心宁神汤/心不藏神不寐/炎症反应/白细胞介素-1β/药效学实验/神经组织病理变化/中医不寐五神分型Key words
Qingxin Ningshen Decoction/insomnia due to heart not storing spirit/inflammatory reaction/interleukin-1β/pharmacodynamics experiment/neuropathological changes/five spirit classification of insomnia in Chinese medicine分类
中医学引用本文复制引用
吴金鸿,张星平,梁政亭,梁瑞宁,陈旭,贾宏林,李振辉,胡燕玲,崔文军..清心宁神汤对心不藏神不寐模型大鼠的药效实验研究[J].湖南中医药大学学报,2025,45(4):588-597,10.基金项目
国家自然科学基金项目(82160873,81960837,81560762,81260526) （82160873,81960837,81560762,81260526）
国家中医药管理局"十四五"高水平中医药重点学科(国中医药人教函[2023]85号) （国中医药人教函[2023]85号）
全国名老中医药专家传承工作室(国中医药人教函[2022]75号) （国中医药人教函[2022]75号）
新疆维吾尔自治区自然科学基金资助项目(2022D01D48) （2022D01D48）
新疆维吾尔自治区自然科学基金(2020D01C163) （2020D01C163）
新疆维吾尔自治区高校科研计划项目(XJEDU2021Y30). （XJEDU2021Y30）
