湖南中医药大学学报2025，Vol.45Issue(4)：647-653,7.DOI:10.3969/j.issn.1674-070X.2025.04.009
去甲乌药碱配伍6-姜酚前后对衰竭心肌细胞影响的研究
Effects of higenamine combined with 6-gingerol on failing cardiomyocytes before and after treatment
摘要
Abstract
Objective To compare the effects of higenamine before and after combination with 6-gingerol on failing cardiomyocytes.Methods A failing H9c2 cell model was established using hydrogen peroxide(H2O2).The cells were divided into nine groups:blank group,model group,6-gingerol group,higenamine single-use groups(5,10,20 μmol/L),and higenamine combined with 6-gingerol(20 μmol/L)groups.The mitochondrial morphology of the cells was observed,and the mitochondrial membrane potential,Ca2+concentration,ATP content,and mitochondrial membrane-associated active transport enzyme activity were measured.Results The replication conditions for inducing failing H9c2 cells were determined as 1000 μmol/L H2O2 for 2 hours.Compared with the model group,both higenamine monotherapy and its combination with 6-gingerol significantly alleviated mitochondrial ultrastructural damage.Compared with the use of higenamine alone,the combination with 6-gingerol had a synergistic protective effect on the model cells,as evidenced by a more intact mitochondrial membrane,clearer boundaries,and restored cristae structure.Additionally,mitochondrial membrane potential was significantly higher,cytoplasmic and mitochondrial Ca2+concentrations were markedly lower,and ATP content,along with the activities of Na+-K+-ATPase,Ca2+-ATPase,and Ca2+-Mg2+-ATPase,significantly enhanced(P＜0.05,P＜0.01).Conclusion The combination of higenamine and 6-gingerol can significantly enhance the protective effect on failing H9c2 cardiomyocytes.This effect may be related to their synergistic action in alleviating mitochondrial calcium overload,increasing mitochondrial ATPase activity,promoting ATP production,and restoring mitochondrial function.关键词
去甲乌药碱/6-姜酚/H9c2心肌细胞/能量代谢/线粒体Key words
higenamine/6-gingerol/H9c2 cardiomyocytes/energy metabolism/mitochondria分类
中医学引用本文复制引用
刘芮祺,李雨晴,肖阳,刘萍,丰正男,傅音睿,王志琪..去甲乌药碱配伍6-姜酚前后对衰竭心肌细胞影响的研究[J].湖南中医药大学学报,2025,45(4):647-653,7.基金项目
湖南省教育厅重点项目(23A0285) （23A0285）
湖南省自然高校联合基金重点项目(2025JJ90002) （2025JJ90002）
湖南省大学生创新创业项目(S202310541091) （S202310541091）
湖南省自然科学基金项目(2021JJ80059) （2021JJ80059）
长沙市自然科学基金项目(kq2014093). （kq2014093）
