解放军医学杂志2025，Vol.50Issue(4)：452-457,6.DOI:10.11855/j.issn.0577-7402.1430.2024.1217
三维光片显微成像在动脉斑块内新生血管可视化及其干预中的应用研究
Three-dimensional light sheet microscopy imaging for evaluating intraplaque neovascularization in arterial plaques and the efficacy of interventions
摘要
Abstract
Objective To investigate application value of three-dimensional light sheet microscopy imaging for evaluating intraplaque neovascularization in arterial plaques and the efficacy of intervention,and to assess the effect of melatonin(MLT)on neovascularization by these means.Methods Thirty-six ApoE-/-model mice were randomly divided into three groups(n=12):vehicle group,MLT group,and MLT+GW9662 intervention group(MLT+GW).The mice were treated with vehicle,MLT alone,or MLT combined with peroxisome proliferator activated receptor-γ(PPARγ)inhibitor GW9662,respectively.The carotid arteries of the models were three-dimensionally imaged using a light sheet microscopy,and the length,volume and other indicators of neovascularization were quantitatively analyzed using Imaris software.Subsequently,CD31 immunohistochemical staining was performed for verification.Results The light sheet microscopy preliminarily achieved the three-dimensional visualization of intraplaque neovascularization,and its structure was observed to be three-dimensionally reticular and scattered.The results of Imaris quantitative analysis showed that,compared with vehicle group,the total intraplaque neovas cularization length in the MLT group was shortened[(15.79±12.90)mm vs.(33.42±11.16)mm,P<0.05],the total volume was reduced[(1.34±1.47)×10-3 mm3 vs.(13.44±7.35)×10-3 mm3,P<0.05],and the volume ratio was decreased(0.44%±0.47%vs.3.76%±1.74%,P<0.05).The above indicators in MLT+GW group were significantly increased compared with those in MLT group[total length:(35.31±4.69)mm,total volume:(8.87±3.46)×10-3 mm3,volume ratio:2.89%±0.38%;P<0.05].The CD31 immunohistochemical staining also supported the above findings(P<0.05).Conclusions Based on the light sheet microscopy imaging technology,the three-dimensional visualization and quantitative analysis of intraplaque neovascularization were preliminarily realized.It was found that MLT could reduce the overall burden of intraplaque neovascularization,and PPARγ might be involved in its regulatory process.关键词
三维成像/动脉粥样硬化/斑块内新生血管Key words
three-dimensional imaging/atherosclerosis/intraplaque neovascularization分类
临床医学引用本文复制引用
江雨凡,马强,佟伟,李越洋,陈韵岱..三维光片显微成像在动脉斑块内新生血管可视化及其干预中的应用研究[J].解放军医学杂志,2025,50(4):452-457,6.基金项目
This work was supported by the National Natural Science Foundation of China(U23A6011,82400060,82300568),and the Youth Innovation Science Foundation of Chinese PLA General Hospital(22QNFC102) 国家自然科学基金(U23A6011,82400060,82300568) （U23A6011,82400060,82300568）
解放军总医院青年自主创新科学基金(22QNFC102) （22QNFC102）
