激光生物学报2025，Vol.34Issue(2)：148-156,9.DOI:10.3969/j.issn.1007-7146.2025.02.007
斑马鱼egr1基因敲除品系的构建及其颅面软骨发育异常分析
Construction of Zebrafish egr1 Gene Knockout Strains and Analysis of Craniofacial Cartilage Developmental Anomalies
摘要
Abstract
The EGR1 protein encoded by early growth response factor 1(egr1)belongs to the Cys2-His2 type zinc finger protein family.Previous studies have demonstrated its critical roles in animal growth,development,reproduction,and immune processes;however,its function in craniofacial development remains unreported.In this study,we utilized in situ hybridization to identify the specific expression of the egr1 gene in the head region of zebrafish during early cartilage development.To elucidate the function of EGR1 in this context,we employed CRISPR/Cas9 gene editing to generate egr1 knockout zebrafish and analyzed their phenotypes.Alcian blue staining revealed abnormalities in craniofacial cartilage development in egr1-/-mutant embryos,including deformities in Meckel's cartilage and ceratohyal,as well as disarray in the opercular and branchial regions.Our findings demonstrated the critical impact of egr1 gene deficiency on early craniofacial cartilage development in zebrafish,offering valuable insights into the pathogenesis of cartilage-related diseases and potential therapeutic strategies.关键词
斑马鱼/egr1/原位杂交/CRISPR/Cas9/软骨发育Key words
zebrafish/egr1/in situ hybridization/CRISPR/Cas9/cartilage development分类
生物学引用本文复制引用
阳剑波,苏娜,赵琳慧,黄淑兰,颜峰,胡翔,刘爱龙,李翔..斑马鱼egr1基因敲除品系的构建及其颅面软骨发育异常分析[J].激光生物学报,2025,34(2):148-156,9.基金项目
长沙市自然科学基金项目(kq2208477) （kq2208477）
国家自然科学基金面上项目(81972642) （81972642）
湖南师范大学大学生创新训练计划项目(2024054). （2024054）
