激光生物学报2025，Vol.34Issue(2)：176-183,192,9.DOI:10.3969/j.issn.1007-7146.2025.02.010
雷帕霉素通过抑制NLRP3炎症小体减轻高糖诱导的足细胞损伤
Rapamycin Alleviates High-sugar Induced Podocyte Damage by Inhibiting the NLRP3 Inflammasome
摘要
Abstract
To investigate rapamycin's effect on high-glucose induced podocyte injury and its related mechanism,human glomerular podocytes(HGPC)were cultured in vitro.The cells were divided into control group,model group,rapamycin group,inhibitor group,rapamycin+inhibitor group and rapamycin+activator group.Podocyte morphology,tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β)levels,cell proliferation rate,apoptosis rate,NOD-like receptor protein 3(NLRP3),apoptosis-associated speck-like protein(ASC),cysteine aspartate protease-1(Caspase-1)mRNA,protein and p38 mitogen-activated protein kinase(p38 MAPK)pathway key protein expression levels were observed.Compared with the control group,the cell arrangement was loose,the cell septum increased,and the cell proliferation rate decreased in the model group(P<0.05),and the levels of TNF-α and IL-1β,apoptosis rate,NLRP3,ASC,Caspase-1 mRNA and protein,and p-p38 MAPK protein increased(P<0.05).Compared with model group,the above cell states and indexes were improved in rapamycin group and inhibitor group(P<0.05).Compared with the rapamycin group,the above cell status and indexes were further improved in the rapamycin+inhibitor group(P<0.05),whereas the opposite was observed in the rapamycin+activator group(P<0.05).Rapamycin can improve the morphology of high glucose induced podiocytes by inhibiting p38 MAPK signaling pathway,promote cell proliferation,inhibit cell apoptosis,inflammatory release and activation of NLRP3 inflammasome,could provide reference for the study of diabetic nephropathy.关键词
糖尿病肾病/足细胞/雷帕霉素/NOD样受体蛋白3炎症小体/p38丝裂原活化蛋白激酶Key words
diabetic nephropathy/podocyte/rapamycin/NOD-like receptor protein 3 inflammasome/p38 mitogen-activated protein kinase分类
临床医学引用本文复制引用
赵学慧,王淮淮,李维维,李迎婕,贾军利..雷帕霉素通过抑制NLRP3炎症小体减轻高糖诱导的足细胞损伤[J].激光生物学报,2025,34(2):176-183,192,9.基金项目
河北省2023年度医学科学研究课题计划项目(20231466) （20231466）
河北省2021年度医学科学研究课题计划项目(20210125). （20210125）
