吉林中医药2025，Vol.45Issue(5)：595-599,5.DOI:10.13463/j.cnki.jlzyy.2025.05.023
祛风扶阳汤降低MPO调节Th17/Treg平衡缓解嗜中性粒细胞哮喘大鼠分子机制研究
The mechanism study of Qufeng Fuyang decoction on ameliorating neutrophilic asthma in a rat model by reducing the level of MPO and balancing the ratio of Th17/Treg
摘要
Abstract
Objective To explore the pharmacological mechanisms of Qufeng Fuyang decoction on reducing the airway hyperreactivity of neutrophil asthma.Methods Thirty female Wistar rats aged 6-7 weeks were randomly divided into 5 groups:a control group,a model group(neutrophil asthma rat model),a positive control group[dexamethasone,oral feeding,2.5 mg/(kg·d)],a Qufeng Fuyang decoction treatment group[oral feeding,100 mg/(kg·d)],a combined treatment group[oral feeding,dexamethasone,2.5 mg/(kg·d)+Qufeng Fuyang decoction,100 mg/(kg·d)],with 6 rats in each group.Bronchoalveolar lavage fluid(BALF)was collected.The levels of myeloperoxidase(MPO),eosinophil cationic protein(ECP),IL-6,IL-8,IL-17 and G-CSF were detected in BALF supernatants.The total number of cells in each view under light microscope with 200×magnification and the number of eosinophilic and neutrophilic granulocytes in every 200 cells were counted in BALF cell smears.The Th17/Treg ratio in BALF cell suspensions was determined by flow cytometry.Results Compared with the control group,the total cell number per view and neutrophil count per 200 cells in BALF smears were increased in the model group,with the proportion of Th17/Treg increased,the level of MPO increased,and the levels of IL-6,IL-8,IL-17 and G-CSF in BALF increased as well(P<0.05).Compared with the model group,the total number of cells per view and neutrophil count per 200 cells in BALF smears were decreased in the combined treatment group,with the Th17/Treg ratio decreased,the MPO level decreased,and the levels of IL-6,IL-8,IL-17 and G-CSF in BALF decreased,too(P<0.05).Compared with the model group,there was no significant difference between the positive treatment group and the Qufeng Fuyang decoction group(P>0.05).The eosinophil count per 200 cells and the ECP level in BALF were not significantly different among all the groups(P>0.05).Conclusion Qufeng Fuyang decoction assists glucocorticoid to regulate the Th17/Treg balance by reducing the level of MPO,so as to reduce the inflammatory cytokines release and alleviate neutrophil asthma in the rat model.关键词
嗜中性粒细胞哮喘/祛风扶阳汤/髓过氧化物酶/Th17/Treg平衡Key words
neutrophil asthma/Qufeng Fuyang decoction/myeloperoxidase/Th17/Treg balance分类
医药卫生引用本文复制引用
范彩虹,李蘩漪,吴玉,姚梓平,辛效毅..祛风扶阳汤降低MPO调节Th17/Treg平衡缓解嗜中性粒细胞哮喘大鼠分子机制研究[J].吉林中医药,2025,45(5):595-599,5.基金项目
新疆维吾尔自治区自然科学基金(2022D01C222) （2022D01C222）
