江苏大学学报（医学版）2025，Vol.35Issue(3)：185-190,6.DOI:10.13312/j.issn.1671-7783.y230228
芸豆激酶粗提物改善小鼠非酒精性脂肪性肝病的作用
Attenuating effect of kidney bean enzyme crude extract on non-alcoholic fatty liver disease in mice
摘要
Abstract
Objective:To investigate the ameliorative effect of kidney bean enzyme crude extract(KBECE)on non-alcoholic fatty liver disease(NAFLD)in mice.Methods:Total of 30 female ICR mice were randomly divided into 10 mice fed with normal diet(normal group),and the other 20 mice were fed with 45%high fat diet to establish the model of NAFLD.After 2 weeks,they were randomly divided into high fat diet control group(high fat group)and KBECE intervention group(intervention group),with 10 mice in each group.The mice were treated with PBS and KBECE daily for 6 weeks,and the body weight was recorded every week.At the 8th week,the mice were sacrificed and the blood and liver tissue were collected.The pathological changes of liver of NAFLD mice were observed by HE and Masson staining.The contents of MDA in liver tissue,TG,TC and LDL-C in serum were measured by commercial kits.Results:KBECE decreased the relative growth rate of body weight of mice,KBECE could improve hepatic steatoid degeneration and fibrosis in hyperlipidemic mice.Compared with the high fat group,the liver index of the intervention group decreased respectively(P<0.05),the content of MDA in liver tissue and the content of TG,TC,LDL-C in serum decreased respectively(all P<0.05).Conclusion:KBECE can improve the NAFLD induced by high fat diet by repairing the disorder of lipid metabolism and inhibiting oxidative stress.关键词
芸豆激酶/非酒精性脂肪性肝病/脂质代谢/氧化应激/粗提物Key words
kidney bean enzyme/non-alcoholic fatty liver disease/lipid metabolism/oxidative stress/crude extract分类
临床医学引用本文复制引用
侯寒进,张俊尧,杨梦婷,李张左,陈茜,邝美倩,龚爱华..芸豆激酶粗提物改善小鼠非酒精性脂肪性肝病的作用[J].江苏大学学报（医学版）,2025,35(3):185-190,6.基金项目
江苏省科技厅社会发展临床前沿技术项目(BE2022778) （BE2022778）
