军事医学2025，Vol.49Issue(4)：250-256,7.DOI:10.7644/j.issn.1674-9960.2025.04.002
Schlafen4在高毒力肺炎克雷伯菌吸入感染致小鼠急性肺炎中的作用
Roles of Schlafen4 in acute pneumonia induced by inhalation infection of hypervirulent Klebsiella pneumoniae
摘要
Abstract
Objective To explore the role of Schlafen4(SLFN4)in acute pneumonia induced by hypervirulent Klebsiella pneumoniae(hvKp)via intratracheal aerosolization.Methods Differential expression gene Slfn4 was identified after infection with hvKp based on RNA sequencing(RNA-seq)and single-cell RNA sequencing(scRNA-seq)data before Slfn4-/-mice were obtained via CRISPR/Cas gene editing technology.Slfn4-/-mice and wild mice were challenged via intratracheal aerosolization.Mortality and weight changes were recorded for 14 d,while pathological changes and expression levels of interleukin-6(IL-6),IL-17A,IL-1β,and tumor necrosis factor-α(TNF-α)were detected at 48 h post-infection.Results SLFN4 expression was significantly increased in wild mice after infection with hvKp.Survival was significantly increased,and weight loss was mitigated before gradual recovery in Slfn4-/-mice after infection.The knockout of SLFN4 attenuated alveolar wall thickening,diminished neutrophil infiltration,and suppressed pro-inflammatory cytokine production(IL-6,IL-17A,IL-1β,TNF-α)in the lung at 48 h post-infection.Conclusion The deletion of SLFN4 may suppress the expression of specific pro-inflammatory cytokines and attenuate neutrophil over-recruitment in the lung,thereby alleviating pneumonia in mice after hvKp infection.关键词
SLFN4/高毒力肺炎克雷伯菌/小鼠/肺炎/中性粒细胞/基因敲除Key words
Schlafen4/hypervirulent Klebsiella pneumoniae/mice/pneumonia/neutrophil/gene knockout分类
基础医学引用本文复制引用
朱小雨,秦佟童,查锦宏,周冬生,杨文慧,高波..Schlafen4在高毒力肺炎克雷伯菌吸入感染致小鼠急性肺炎中的作用[J].军事医学,2025,49(4):250-256,7.基金项目
病原微生物生物安全国家重点实验室自主研究课题(SKLPBS2225) （SKLPBS2225）
