菌物学报2025，Vol.44Issue(5)：1-14,14.DOI:10.13346/j.mycosystema.250009
暗褐脉柄牛肝菌种质资源遗传多样性
Genetic diversity of Phlebopus portentosus germplasm resources
摘要
Abstract
The population genetic structure and genetic diversity of Phlebopus portentosus germplasm resources of 53 samples from 9 regions in southern China were identified by using rDNA ITS and SSR molecular markers.The results showed that all 53 samples were Phlebopus portentosus,and the similarity of their ITS sequences was 92.09％.In total,252 polymorphic loci were successfully amplified by 28 pairs of SSR primers,with each pair exhibiting 48-53 amplification sites and the polymorphism rate exceeding 90％.The Nei's genetic diversity index and Shannon's diversity index were determined to be 0.371 6-0.605 1 and 0.327 3-0.584 5,respectively.At the population level,Nei's genetic diversity index and Shannon's diversity index were 0.097 3-0.237 0 and 0.142 0-0.358 6,respectively,and the polymorphism rate was 23.48％-74.09％,indicating abundant genotypes within populations.The average genetic differentiation coefficient and gene flow between populations were 0.282 0 and 1.273 2 respectively,indicating a low degree of genetic differentiation and gene exchange.The genetic similarity coefficient of the UPGMA cluster ranged from 0.67 to 0.89,and the 53 samples could be divided into 3 groups at the level of 0.72,showing basically consistent with the results of the PCoA principal component analysis,the Mantel test and the STRUCTURE clustering.The application of population structure analysis enabled the division of samples into 8 genetic components,with subgroup 2 being identified as the most abundant in terms of genotypes.AMOVA analysis revealed that 90％of the observed genetic variation could be ascribed to within-population genetic factors.The tested population of Phlebopus portentosus exhibited a substantial number of genotypes,a moderate degree of genetic differentiation,and impeded gene exchange.关键词
暗褐脉柄牛肝菌/遗传多样性/SSR分子标记Key words
Phlebopus portentosus/genetic diversity/SSR molecular marker引用本文复制引用
李凡,曹瑶,杨爱霞,罗祥英,雷洪涛,孟国良,董彩虹,李荣春..暗褐脉柄牛肝菌种质资源遗传多样性[J].菌物学报,2025,44(5):1-14,14.基金项目
云南省(珍稀食用菌)企业技术中心专项资金(JSJ2023016KJ) This work was supported by the Special Fund of Yunnan Province(Rare Edible Fungi)Enterprise Technology Center(JSJ2023016KJ). （珍稀食用菌）
