菌物学报2025，Vol.44Issue(5)：30-40,11.DOI:10.13346/j.mycosystema.240267
一株野生狄更斯邦氏孔菌的生物学特性及驯化栽培
Biological characteristics and domestic cultivation of a wild Bondarzewia dickinsii strain
摘要
Abstract
Macrofungal germplasm resources are an important part of the construction of national germplasm banks.A wild fungal strain collected from Baishanzu National Nature Reserve was isolated and purified,and it was identified as Bondarzewia dickinsii by morphology and ITS sequence analysis.The effects of different carbon sources,nitrogen sources,pH,temperature and illumination on the mycelium growth under solid culture conditions were studied by cross streaking method.Three optimal levels were selected from the above four single factor experiments for orthogonal test.The results showed that the optimal carbon source was starch;the optimal nitrogen source was yeast extract;the optimal pH was 7.0,and the optimal temperature was 25 ℃.The fruiting bodies of Bondarzewia dickinsii were obtained by domestication and cultivation,and the content of crude polysaccharide,crude protein,and 16 amino acids in the fruiting bodies were determined.The total polysaccharide content is as high as 37.29 g/100 g,and the crude protein content 24.9 g/100 g.This study provides a reference for the further research and development of this fungus.关键词
狄更斯邦氏孔菌/种质资源/生物学特性/驯化栽培Key words
Bondarzewia dickinsii/germplasm resources/biological characteristics/domestication and cultivation引用本文复制引用
陈俊良,杨应昆,吴国锋,刘朴,李玉..一株野生狄更斯邦氏孔菌的生物学特性及驯化栽培[J].菌物学报,2025,44(5):30-40,11.基金项目
浙江省科技部食用菌新品种选育项目(2021C02073) This work was supported by the New Variety Breeding Project of Science Technology Department of Zhejiang Province,China(2021C02073). （2021C02073）
