菌物学报2025，Vol.44Issue(5)：41-49,9.DOI:10.13346/j.mycosystema.240335
产胞外多糖粒毛盘菌属真菌菌株的筛选及发酵营养配方优化
Screening of exopolysaccharide-producing strains of the genus Lachnum and optimization for their fermentation nutrient formula
摘要
Abstract
Thirteen strains of the genus Lachnum producing extracellular polysaccharide were isolated from the roots of Betula ermanii in the tundra of Changbai Mountains.A high-yield strain L01 was screened out by measuring the yield of crude extracellular polysaccharide.The results of single factor test and response surface analysis showed that the optimal fermentation medium was composed of 13.57 g/L soluble starch as carbon source,15.23 g/L cornmeal as nitrogen source,and 6.86 g/L NaCl as inorganic salt source.Under these nutrient conditions,the extracellular polysaccharide yield of the strain L01 was 1.941 mg/mL.The significance for the effects of the tested factors on the exopolysaccharide production of strain L01 was graded in the order of soluble starch＞NaCl＞cornmeal.This study provides reference material for in-depth study and resource utilization of extracellular polysaccharides produced by Lachnum fungi.关键词
粒毛盘菌属/产胞外多糖菌株/响应面法/发酵条件优化Key words
Lachnum/exopolysaccharide-producing strain/response surface method/optimization of fermentation condition引用本文复制引用
许榕凡,孙晓东,李琳琳,苏丹,戴冠华,吕国忠,杨红..产胞外多糖粒毛盘菌属真菌菌株的筛选及发酵营养配方优化[J].菌物学报,2025,44(5):41-49,9.基金项目
国家自然科学基金(31170020,31770025) （31170020,31770025）
中央高校基本科研业务费项目 This work was supported by the National Natural Science Foundation of China(31170020,31770025)and the Fundamental Research Funds for the Universities. （31170020,31770025）
