菌物学报2025，Vol.44Issue(5)：97-115,19.DOI:10.13346/j.mycosystema.240274
大肥菇胞内多糖体外低氧模型的条件优化及其酵解特征
Condition optimization of hypoxia model in vitro and glycolysis characteristics of intracellular polysaccharide from Agaricus bitorquis
摘要
Abstract
The intracellular polysaccharide(IPS)of Agaricus bitorquis has excellent anti-hypoxia activity,but its molecular weight is too large to be directly absorbed by human intestine.In order to further study the anti-hypoxia mechanism of intracellular polysaccharide from A.bitorquis at the small molecule level,in vitro model based on the intestinal flora of male and female mice fed under hypoxic conditions was constructed,and the glycolysis conditions were optimized.The enzymatic characteristics of IPS in the intestinal flora of different genders were analyzed.The optimal glycolysis condition in vitro for the female group(group M)was fermentation time of 37 h,initial IPS content of 24.0 mg/mL,bacterial concentration to medium ratio of 6∶4 and bacterial community concentration of 30％,and that for the male group(group F)was fermentation time of 39 h,initial polysaccharide content of 26.0 mg/mL,bacterial community to medium ratio of 8∶5 and bacterial community concentration of 30％.At the end point of glycolysis,the total and reducing sugar consumption rates of group M and group F were higher than 90％,and nine monosaccharides and uronic acids(MSUA)were produced by polysaccharide degradation,including glucose,glucuronic acid,mannose,galactose,galacturonic acid,fructose,ribose,xylose and arabinose.In group M,the monosaccharides were mainly glucuronic≥xylose＞fructose.In group F,they were mainly mannose＞fructose＞galacturonic acid.The total amount of MSUA in group F was nearly 1 times higher than that in group M.Six short-chain fatty acids(SCFAs)were also generated,namely acetic acid,propionic acid,isobutyric acid,butyric acid,isovaleric acid and valeric acid,but acetic acid,butyric acid,propionic acid and isovaleric acid were in chief in both groups M and F.The content of acetic acid in group F was much higher than that of the other three fatty acids,and the content of butyric acid in group M was higher than that in group F.The composition of hypoxic intestinal flora in groups M and F was similar,but the relative proportions of each floristic component were different.Polysaccharide promoted the relative abundance of Bifidobacteria and Prevotella in group M,and significantly increased the relative abundance of Prevotella and Rossella in group F,while decreased the relative abundance of Escherichia coli and Shigella in groups M and F.It was found that compared with the hypoxic intestinal flora of female mice,the hypoxic intestinal flora of male mice showed better activity in degrading intracellular polysaccharide in the in vitro glycolysis model,providing a new idea for the study of simulated polysaccharide digestion.关键词
胞内多糖/大肥菇/体外低氧模型/条件优化/酵解特征Key words
intracellular polysaccharide/Agaricus bitorquis/hypoxia model in vitro/condition optimization/glycolysis characteristics引用本文复制引用
邱松林,胡世成,童彩玲,焦迎春..大肥菇胞内多糖体外低氧模型的条件优化及其酵解特征[J].菌物学报,2025,44(5):97-115,19.基金项目
国家自然科学基金地区项目(31960471) This work was supported by the Regional Project of National Natural Science Foundation of China(31960471). （31960471）
