临床传统医学和药理学（英文）2025，Vol.6Issue(1)：14-24,11.DOI:10.1016/j.ctmp.2024.200185
红景天通过HIF-1α/TGF-β/Smad信号通路改善乳腺癌免疫抑制微环境
Rhodiola rosea L.improves the immunosuppressive microenvironment through the HIF-1α/TGF-β/Smad signaling pathway in breast cancer
摘要
Abstract
Background:Rhodiola rosea L.(R.rosea),a traditional Chinese medicinal herb,has gained global attention for its diverse pharmacological activities,especially its anti-tumor effect.While studies have confirmed that R.rosea can hinder the growth of multiple cancer cells,the underlying mechanisms of R.rosea in breast cancer remain elusive. Objective:To investigate the anti-breast cancer efficacy and potential mechanism of R.rosea. Methods:UPLC-Q/TOF MS was utilized to examine the components of R.rosea.Eight-week-old MMTV-PyMT mice were used to assess the potential impact of R.rosea in breast cancer development.Flow cytometry was performed to evaluate the effectiveness of immune cells in mediating the anti-tumor effects of R.rosea.Additionally,the xCELLigence system aided in examining the proliferation of breast cancer cells after treatment with R.rosea.Furthermore,RNA-seq,RT-qPCR and Western blot were employed to elucidate the underlying mechanism of R.rosea in breast cancer.Lastly,network pharmacology was applied to delve deeper into the primary chemically active components responsible for the pharmacological effects of R.rosea. Results:R.rosea exhibits a substantial effect in curtailing the progression of breast cancer,accomplished by repressing the proliferation of tumor cells and reconfiguring the tumor microenvironment(TME).Mechanistically,R.rosea exerts its anti-tumor effects by downregulating the expression of HIF-1α,TGF-β,and Smad3 in tumor cells,thereby alleviating the hypoxic TME and enhancing immune surveillance. Conclusion:Our study revealed that R.rosea suppressed the development of breast cancer by inhibiting the hy-poxic immune microenvironment mediated by the activation of HIF-1α/TGF-β/Smad signaling pathway in tumor cells.These findings,which underscore R.rosea potential as a potent antineoplastic agent,offer a compelling basis for its use as an adjuvant therapy in the treatment of breast cancer.关键词
红景天/缺氧诱导因子-α/肿瘤微环境/炎症/乳腺癌Key words
Rhodiola rosea L./Hypoxia-inducible factor-l alpha/Tumor microenvironment/Inflammation/Breast cancer引用本文复制引用
白宁宁,傅惠英,郭迎雪,周美玲,陈江琳,林晓晨,刘霞,金璐,周秋景,寿旗扬..红景天通过HIF-1α/TGF-β/Smad信号通路改善乳腺癌免疫抑制微环境[J].临床传统医学和药理学（英文）,2025,6(1):14-24,11.基金项目
This research was sponsored by the National Natural Science Foun-dation of China(Grant 82274175 and 82474168 to Q.Y.S.,Grant 82174026 to H.Y.F.,Grant 82404915 to L.J.),the Natural Science Foun-dation of Zhejiang Province(Grant LY22H280012 to H.Y.F..,Grant Y23H280029 to X.L.),Zhejiang Provincial Administration of Traditional Chinese Medicine Co-construction Key Project(Grant GZY-ZJ-KJ-23069 to Q.Y.S.),and Zhejiang Provincial Program for the Cultivation of High-level Innovative Health talents. （Grant 82274175 and 82474168 to Q.Y.S.,Grant 82174026 to H.Y.F.,Grant 82404915 to L.J.）
