Saba senegalensis(A.DC.)Pichon(夹竹桃科)提取物的分子网络法化学分析及其对秀丽隐杆线虫的驱虫活性
Mohamed B.Belemlilga Philippe M.Loiseau Pierre Champy Innocent P.Guissou Hazrina Hazn Aristide Traore Indira Dennemont Sébastien Pomel Sandrine Cojean Mehdi A.Beniddir Sylvin Ouedraogo Christian Bories
临床传统医学和药理学（英文）2025，Vol.6Issue(1)：50-62,13.
临床传统医学和药理学（英文）2025，Vol.6Issue(1)：50-62,13.DOI:10.1016/j.ctmp.2025.200195
Saba senegalensis(A.DC.)Pichon(夹竹桃科)提取物的分子网络法化学分析及其对秀丽隐杆线虫的驱虫活性
Chemical analysis by molecular networking methods on extracts of Saba senegalensis(A.DC.)Pichon(Apocynaceae)and its anthelmintic activity on Caenorhabditis elegans
摘要
Abstract
Background:Drug resistance is increasing in gastrointestinal parasitosis;it is therefore essential to identify new anthelmintic molecules. Objective:The study aims to evaluate the anthelmintic activity of extracts and fractions of leaves of Saba sene-galensis(A.DC.)Pichon(Apocynaceae)against L3 larvae of Caenorhabditis elegans,a free-living helminth model and to highlight potential anthelmintic molecules by dereplication strategy of molecular networking. Methods:The methanolic extract,ethanolic extract and aqueous extract of the plant's leaves were carried out,followed by flash chromatography on silica gel for by fractionation,HPLC-MS/MS analysis.The tandem mass data were used for generation of molecular network.The anthelmintic effects of aqueous and hydro-ethanolic fractions of leaves of S.senegalensis on Caenorhabditis elegans worm viability were carried out by using both 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-tetrazolium bromide(MTT)assay and microscope motility observation. Results:Among the aqueous fractions,the aqueous decoction showed an EC50 at 0.24 mg/ml and a viability rate of 7.5％at 0.9 mg/ml.In hydro-ethanolic extracts,hydro-ethanolic macerate residual(Fe-3)exhibited a significant effect with an EC50 at 0.29 mg/ml and 12.5％of viability at 0.9 mg/ml.HPLC-ESI-MS/MS analysis allowed the identification of twelve compounds. Conclusions:Using the MTT-formazan assay,extracts of S.senegalensis exhibit a moderate anthelmintic effect on C.elegans worms linked to natural antiparasitic molecules.关键词
Saba senegalensis/秀丽隐杆线虫/分子网络/ESI HPLC(+/-)MS/MSKey words
Saba senegalensis/Caenorhabditis elegans/Molecular networking/ESI HPLC(+/-)MS/MS引用本文复制引用
Mohamed B.Belemlilga,Philippe M.Loiseau,Pierre Champy,Innocent P.Guissou,Hazrina Hazn,Aristide Traore,Indira Dennemont,Sébastien Pomel,Sandrine Cojean,Mehdi A.Beniddir,Sylvin Ouedraogo,Christian Bories..Saba senegalensis(A.DC.)Pichon(夹竹桃科)提取物的分子网络法化学分析及其对秀丽隐杆线虫的驱虫活性[J].临床传统医学和药理学（英文）,2025,6(1):50-62,13.基金项目
This work was done with the University of Paris Saclay,with finan-cial support from Campus France(895739H)through the French Em-bassy in Burkina Faso. （895739H）
