临床传统医学和药理学（英文）2025，Vol.6Issue(1)：63-72,10.DOI:10.1016/j.ctmp.2025.200196
桑色素通过抑制氧化应激与炎症反应改善小鼠创伤后应激及酒精使用障碍共病中的心肾系统损伤
Morin inhibits oxidative stress and inflammation in the cardiorenal system associated with post-traumatic stress and alcohol use disorders in mice
Benneth Ben-Azu 1Adrian I.Omogbiya 1Jerome N.Asiwe 2Benjamin Oritsemuelebi 1Emmanuel O.Chidebe 1Jackson E.Onuelu 1Happy Isibor 1Orovwigho Ogheneoruese 1Winifred E.Demaki 1Solomon O.Otuacha1
作者信息
- 1. DELSU Joint Canada-Israel Neuroscience and Biopsychiatry Laboratory Department of Pharmacology Faculty of Basic Medical Sciences,Delta State University,Abraka 330105,Nigeria
- 2. Department of Physiology,Faculty of Basic Medical Sciences,Delta State University,Abraka 330105,Nigeria
- 折叠
摘要
Abstract
Background:Alcohol use and traumatic events have a connecting relationship owing to the rising number of people adopting alcoholism as a coping strategy.
Objectives:This study examined the cardiorenal protective effect of morin,a bioflavonoid,in mice comorbidly exposed to alcohol use disorder(AUD)and posttraumatic stress disorder(PTSD).
Methods:Mice exposed to single-prolong-stress(SPS)-induced PTSD were submitted to every-other-day ethanol(2 g/kg,oral gavage)for AUD induction,alongside treatments with morin(50 mg/kg and 100 mg/kg)or fluox-etine(10 mg/kg),from days 8-21 once daily.After that,mice were euthanized on day 22,markers of oxidative stress(glutathione,catalase,superoxide dismutase,malondialdehyde,nitrite)and inflammatory cytokines(tumor necrosis factor(TNF-α),interleukin-6(IL-6))in the kidney and heart were assayed.
Results:Our result showed that mice exposed to SPS+EtOH-induced PTSD-AUD had reduced levels of glutathione,catalase,and superoxide dismutase,with increased malondialdehyde and nitrite concentrations in the heart and kidney relative to SPS+EtOH group.Also,the SPS+EtOH group showed increased concentrations of TNF-α and IL-6 in the heart and kidney tissues,suggesting inflammatory activity relative to normal control.Treatment with morin(50 mg/kg and 100 mg/kg)significantly reduced the SPS-EtOH-induced oxidative and nitrergic stress relative to the SPS+EtOH group.Additionally,the increased release of TNF-α and IL-6 following PTSD-AUD induction was profoundly inhibited by morin in a similar manner to fluoxetine.
Conclusion:Our findings suggest that AUD-PTSD interaction-induced organ dysfunction,such as cardiorenal impairments,was reversed by morin via mechanisms associated with attenuation of oxidative/nitrergic stress and release of pro-inflammatory cytokines in mice.关键词
酒精使用障碍/创伤后应激障碍/心肾功能障碍/氧化应激/抗氧化剂/桑色素Key words
Alcohol use disorder/Post-traumatic stress disorder/Cardiorenal dysfunction/Oxidative stress/Antioxidants/Morin引用本文复制引用
Benneth Ben-Azu,Adrian I.Omogbiya,Jerome N.Asiwe,Benjamin Oritsemuelebi,Emmanuel O.Chidebe,Jackson E.Onuelu,Happy Isibor,Orovwigho Ogheneoruese,Winifred E.Demaki,Solomon O.Otuacha..桑色素通过抑制氧化应激与炎症反应改善小鼠创伤后应激及酒精使用障碍共病中的心肾系统损伤[J].临床传统医学和药理学（英文）,2025,6(1):63-72,10.
