临床传统医学和药理学（英文）2025，Vol.6Issue(1)：73-83,11.DOI:10.1016/j.ctmp.2025.200197
综合体外、体内及计算机模拟法探讨Baccaurea motleyana的治疗潜力
Investigating the therapeutic potential of Baccaurea motleyana through an integrated approach utilizing in vitro,in vivo,and in silico investigations
摘要
Abstract
Background:Baccaurea motleyana Müll.Arg.,commonly known as Rambai,is a fruit-bearing tree with potential nutraceutical and remedial properties.Traditionally,it is used for postpartum recovery and eye ailments. Objective:This research intended to explore the pharmacological benefits of the methanol extract of Baccaurea motleyana leaves(MEBML)using a comprehensive approach. Methods:The efficacy of the methanol extract of Baccaurea motleyana leaves(MEBML)at 200 mg/kg and 400 mg/kg was evaluated through various in vivo models,including antipyretic testing in Swiss albino mice,analgesic effects via acetic acid-induced writhing and formalin-induced paw licking,and antidiarrheal activity through castor oil-induced diarrhea and gastrointestinal motility tests.Additionally,in vitro cytotoxic effects were assessed using the brine shrimp lethality bioassay,while computational analysis explored interactions between MEBML phytochemicals and proteins related to target diseases. Results:The methanol extract of Baccaurea motleyana leaves(MEBML)demonstrated a significant reduction in yeast-induced pyrexia in Swiss albino mice.It also exhibited a dose-dependent inhibition in both the acetic acid-induced writhing and formalin-induced paw-licking tests,highlighting its analgesic potential.MEBML showed noteworthy antidiarrheal activity in the castor oil-induced diarrhea model and displayed a cytotoxic effect with an LC50 value of 125.6 yg/ml.Furthermore,phytochemicals from MEBML exhibited strong binding affinities,ranging from-6.1 kcal/mol to-7.3 kcal/mol,with key drug target receptors.These include cyclooxygenase-1(PDB ID:2OYE)and cyclooxygenase-2(PDB ID:6COX)for analgesic and antipyretic activities,the M3 muscarinic cholinergic receptor(PDB ID:5ZHP)for antidiarrheal effects,and human cytochrome P450 2C9(PDB ID:1OG5)for cytotoxic evaluations. Conclusion:In conclusion,MEBML showcased substantial pharmacological activity in pain relief,antipyretic ef-fects,antidiarrheal action,and cytotoxicity in diverse experimental systems,indicating its promise as a foundation for novel drug development.关键词
Baccaurea motleyana/解热/镇痛/止泻/替代医学/计算机模拟Key words
Baccaurea motleyana/Antipyretic/Analgesic/Antidiarrheal/Alternative medicine/In silico引用本文复制引用
Jarin Tasnim Sany,Liakot Ali,Ekramul Haque Ekram,Tanveer Ahsan..综合体外、体内及计算机模拟法探讨Baccaurea motleyana的治疗潜力[J].临床传统医学和药理学（英文）,2025,6(1):73-83,11.基金项目
This research was generously funded and supported by the Na-tional Science and Technology(NST)fellowship program of the Ministry of Science and Technology,Government of the People's Republic of Bangladesh(Award Id:120005100-3821117). （NST）
