临床口腔医学杂志2025，Vol.41Issue(5)：276-279,4.DOI:10.3969/j.issn.1003-1634.2025.05.005
含氟涂料对学龄前儿童口腔微生物多样性的短期影响
Short-term effects of fluoride-containing coatings on oral microbial diversity in preschool children
摘要
Abstract
Objective:To investigate the short-term effects of fluoride varnish on the oral microbial diversity,commu-nity structure,and functional genes of preschool children with varying caries levels,thereby providing evidence for personal-ized caries prevention strategies.Methods:Sixty 36-month-old children were recruited and categorized into no caries,low caries,and high caries groups.Supragingival plaque samples were collected at baseline,Day 1,Day 3,and Day 7 post-fluoride application.Microbial diversity,community structure,and functional gene changes were analyzed using 16S rRNA high-throughput sequencing.Results:After fluoride application,the diversity of microbiomes significantly decreased in all groups(P＜0.01),with the high-caries group exhibiting the greatest change and the slowest dynamic adjustment of microbiome di-versity and structure.Principal component analysis revealed that the community structure of the high-caries group deviated the farthest from baseline on the 1st day post-fluoride application(P＜0.01),and on the 7th day,the diversity and structure of the microbiome had not fully returned to pre-application status(P＜0.05).Functional prediction analysis indicated that the abun-dance of sugar metabolism-related genes in the high-caries group was significantly higher than in the other groups at baseline(P＜0.001),which decreased significantly post-fluoride application(P＜0.01),while the abundance of fluoride resistance genes was significantly upregulated(P＜0.001).Conclusion:The short-term effects of fluoride varnish on oral microbial com-munities vary significantly by caries level,highlighting the necessity for personalized caries prevention strategies and guiding future multi-omics research.关键词
含氟涂料/口腔菌群/多样性/糖代谢/氟抗性基因/龋病防控Key words
Fluoride varnish/Oral microbiota/Diversity/Sugar metabolism/Fluoride resistance genes/Caries pre-vention分类
口腔医学引用本文复制引用
马洪超..含氟涂料对学龄前儿童口腔微生物多样性的短期影响[J].临床口腔医学杂志,2025,41(5):276-279,4.基金项目
河南省高等学校重点科研项目(编号:24B320014) （编号:24B320014）
