岭南现代临床外科2025，Vol.25Issue(2)：75-83,9.DOI:10.3969/j.issn.1009-976X.2025.02.001
FATP1调控的脂肪酸代谢促进胆管癌淋巴结定植
Fatty acid metabolism regulated by FATP1 promotes lymph node colonization in cholangiocarci-noma
摘要
Abstract
Objective To investigate the mechanism and therapeutic strategy of fatty acid transport protein 1(FATP1)on lymph node colonization of cholangiocarcinoma(CCA).Methods Subcutaneous tumor-bearing mouse models and lymph node orthotopic tumor-bearing mouse models were established.Tumor tissues at different tumor stages were collected,and proteomics was used to screen key proteins in cholangiocarcinoma colonization in lymph nodes.Immunohistochemical staining was used to evaluate the correlation between FATP1 expression in lymph node metastases of cholangiocarcinoma and poor progno-sis of patients.CCK-8 cell proliferation assay and Nile red staining were used to detect the changes in lipid metabolism and proliferation ability of cholangiocarcinoma cells(HUCCT1,HCCC9810)before and after knockdown or overexpression of FATP1.In vitro and in vivo experiments were used to verify that FATP1 inhibitor(FATP1-IN-1)inhibited cholangiocarcinoma colonization in lymph nodes by targeting lipid metabolism.Results Proteomic screening results suggested that FATP1 played a key role in the process of cholangiocarcinoma colonization of lymph nodes.Immunohistochemical staining showed that FATP1 was more highly expressed in lymph node metastases and was associated with poor prognosis of patients.In vitro experiments showed that knocking down FATP1 inhibited cholangiocarcinoma prolifera-tion and lipid accumulation,while overexpression of FATP1 enhanced the above phenotypes.FATP1 inhibitor(FATP1-IN-1)significantly inhibited cancer cell proliferation in vitro and reduced tumor burden and lipid accumulation in an in vivo model.Conclusion Fatty acid metabolism regulated by FATP1 promotes cholangiocarcinoma colonization in lymph nodes,and targeting FATP1 can effectively inhibit the progression of lymph node colonized tumors.关键词
胆管癌/淋巴结转移/转移定植/FATP1/FATP1-IN-1Key words
cholangiocarcinoma/lymph node metastasis/metastatic colonization/FATP1/FATP1-IN-1分类
临床医学引用本文复制引用
李修贤,宋之潇,张洪华,刘超..FATP1调控的脂肪酸代谢促进胆管癌淋巴结定植[J].岭南现代临床外科,2025,25(2):75-83,9.基金项目
广东省基础与应用研究基金(2023A1515220141) （2023A1515220141）
博士后科学基金(2024M753783 （）
GZB20240905) （）
