现代畜牧兽医Issue(4)：8-13,6.DOI:10.20154/j.cnki.issn1672-9692.2025.04.002
利用仿生矿化方法提高戊型肝炎假病毒颗粒的热稳定性
Biomimetic mineralization was used to improve the thermal stability of hepatitis E virus-like particles
摘要
Abstract
The study aims to enhance the thermostability of hepatitis E pseudovirus particles(MS2-HEV)using a bionically mineralized method to address the heat resistance issue of hepatitis E virus(HEV)nucleic acid testing quality control samples.The recombinant plasmid pET28b-MS2-HEV was transformed into Rosetta(DE3)supercompetent cells,and MS2-HEV was prepared after induction with isopropyl-β-D-thiogalactopyranoside(IPTG).MS2-HEV was then placed in a mineralization solution with a pH of 7.5,a final calcium ion(Ca²⁺)concentration of 5.5 mmol/L,and a calcium-to-phosphorus ratio(Ca/P)of 1.67,and mineralized at 37℃for 3 hours to prepare bionically mineralized hepatitis E pseudovirus particles(MS2-HEV-CaP).The mineralization efficiency and thermostability were analyzed using RT-qPCR and electron microscopy.The results showed that the mineralization efficiency of MS2-HEV reached 70%,and the mineralized MS2-HEV-CaP could be stably stored at 37℃for 14 days.The study demonstrated that MS2-HEV-CaP was successfully prepared,and the thermostability of MS2-HEV was improved using the bionically mineralized method.关键词
戊型肝炎/假病毒颗粒/仿生矿化/热稳定性Key words
Hepatitis E/virus-like particles/Biomimetic mineralization/Thermal stability分类
农业科技引用本文复制引用
朱志梅,杨书仁,许艺凡,李慧,王术德,高慎阳..利用仿生矿化方法提高戊型肝炎假病毒颗粒的热稳定性[J].现代畜牧兽医,2025,(4):8-13,6.基金项目
辽宁省教育厅面上项目(JYTMS20231735) （JYTMS20231735）
辽宁省科学技术计划农业重大专项(2023JH110200006) （2023JH110200006）
