现代畜牧兽医Issue(4)：14-18,5.DOI:10.20154/j.cnki.issn1672-9692.2025.04.003
BHK-21细胞悬浮培养新城疫病毒工艺初步研究及免疫效果评价
Study on the suspended cell culture-based Newcastle disease virus in BHK-21 and immunogenicity evaluation
摘要
Abstract
This study aimed to establish an efficient suspension culture process for Newcastle disease virus(NDV)strain A-Ⅶ in BHK-21 cells and evaluate its immunogenicity.Key parameters,including initial cell density,harvest time,virus-to-cell ratio,and cultivation temperature,were optimized to enhance viral production.Subsequently,the immunogenicity of the vaccine produced under optimized conditions was assessed.Results demonstrated that the highest viral yield was achieved under the following optimal conditions:initial BHK-21 cell density of 4.12×10⁶ cells/mL,virus-to-cell volume ratio of 0.02%,and harvest at 72 hours post-inoculation with cultivation at 35℃.The hemagglutination(HA)titer of the suspension-cultured virus reached 1:512.Comparative immunogenicity analysis in SPF chickens revealed that the hemagglutination inhibition(HI)antibody titers induced by the suspension-cultured virus were significantly lower than those elicited by the egg-cultured virus(P<0.05).These findings suggest that the suspension culture process holds significant potential for NDV strain A-Ⅶ production,but further optimization is required to improve its immunogenicity and enhance antibody response levels.关键词
BHK-21细胞/悬浮培养/新城疫病毒/免疫效果Key words
BHK-21/Suspension culture/Newcastle disease virus/Immunogenicity分类
农业科技引用本文复制引用
任培森,吕文静,刘学,尹喆,黄金海,刘海霞,李伊铭..BHK-21细胞悬浮培养新城疫病毒工艺初步研究及免疫效果评价[J].现代畜牧兽医,2025,(4):14-18,5.基金项目
天津市科技支撑重点项目(22YFZCSN00080) （22YFZCSN00080）
