摘要
Abstract
This study aimed to systematically reveal the molecular characteristics of the M protein of swine acute diarrhea syndrome coronavirus(SADS-CoV),providing references for vaccine design and antiviral drug development.Using bioinformatics methods,the structural and immunofunctional properties of the SADS-CoV M protein were systematically analyzed.Results showed that the SADS-CoV M protein consists of 228 amino acids,containing three transmembrane domains without a signal peptide,and exhibits hydrophobic stability.The secondary structure of the SADS-CoV M protein is predominantly composed of α-helices(32.89%)and random coils(38.60%),the predicted tertiary structure exhibits a 34.31%similarity to that of the SARS-CoV-2 M protein.Based on the tertiary structure,B-cell epitope prediction analysis was performed,identifying four high-probability B-cell epitopes(positions 13～23,65～73,80～88,and 188～195),suggesting potential targets for immune intervention.The study demonstrated that the SARS-CoV-2 M protein exhibits molecular stability and strong immunogenicity,providing a molecular foundation for the development of novel vaccines.关键词
猪急性腹泻综合征冠状病毒/M蛋白/生物信息学/抗原表位Key words
Swine acute diarrhea syndrome coronavirus/M protein/Bioinformatics/Antigenic epitopes分类
农业科技
