现代畜牧兽医Issue(4)：24-27,4.DOI:10.20154/j.cnki.issn1672-9692.2025.04.005
猪肺炎支原体Y株培养基额外添加碳源含量的筛选
Screening of additional carbon source content in the culture medium for Mycoplasma hyopneumoniae strain Y
郑鑫 1刘超 1刘艳霞 1侯芳芳 1吕磊 1曹志丹 1舒秀伟1
作者信息
- 1. 辽宁益康生物股份有限公司,辽宁 辽阳 111000
- 折叠
摘要
Abstract
The aim of this experiment was to improve the growth titer of Mycoplasma hyopneumoniae strain Y,and to provide a reference for the development and production of porcine mycoplasmal pneumonia vaccine.In this experiment,glucose was used as the carbon source of culture medium,and culture medium with different glucose content(1.25,5.00,7.50,10.00,12.50 g/L)was selected for resuscitation and transmission of Mycoplasma hyopneumoniae strain Y.The color change unit(CCU)counting test was performed on the bacterial solution of different generations.The results showed that the CCU count of Mycoplasma hyopneumoniae strain Y was the highest when the glucose content was 7.50 g/L,and the CCU count of each growth generation in this group was the highest among the five groups of the same generation.In all generations,the lowest CCU counts were in Group 1(medium glucose content 1.25 g/L)and Group 5(medium glucose content 12.50 g/L).The results showed that the most suitable medium for the growth of Mycoplasma hyopneumoniae strain Y was 7.50 g/L glucose content,and the CCU count of the resuscitated and transcultured Mycoplasma hyopneumoniae strain Y was significantly higher than that of other groups,and the growth state was relatively stable.关键词
猪肺炎支原体/碳源/生长滴度/猪支原体肺炎Key words
Mycoplasma hyopneumoniae/Carbon source/Growth titer/Porcine mycoplasmal pneumonia分类
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郑鑫,刘超,刘艳霞,侯芳芳,吕磊,曹志丹,舒秀伟..猪肺炎支原体Y株培养基额外添加碳源含量的筛选[J].现代畜牧兽医,2025,(4):24-27,4.
