现代畜牧兽医Issue(4)：28-32,5.DOI:10.20154/j.cnki.issn1672-9692.2025.04.006
角蛋白酶对牛血液蛋白的酶解效果研究
Study on the enzymatic hydrolysis effect of keratinase on bovine blood proteins
摘要
Abstract
The study conducted research on the enzymatic hydrolysis of livestock blood to provide a reference for the rational treatment of poultry and livestock blood.Using bovine blood as the sample,keratinase was employed for blood enzymatic hydrolysis experiments.Four factors-Sample pH,enzyme dosage,hydrolysis temperature,and hydrolysis time-were investigated through single-factor and orthogonal experiments to examine their effects on the degree of hydrolysis under different enzymatic conditions and optimize the process parameters.The results showed that the optimal enzymatic hydrolysis conditions for bovine blood protein were:pH 9,enzyme dosage 3.75％,temperature 65℃,and duration 28 hours,achieving a hydrolysis degree of 49.66％ under these conditions.The study demonstrates that keratinase exhibits favorable enzymatic hydrolysis effects on bovine blood protein,providing valuable insights for rational recycling of livestock blood,reducing resource waste,and alleviating environmental pollution issues.关键词
角蛋白酶/家畜血液/水解度/酶解Key words
Keratinase/Livestock blood/Degree of hydrolysis/Enzymatic hydrolysis分类
农业科技引用本文复制引用
李金浩,文涛,李岩,高钰涵,蓝本翔,周雪雁..角蛋白酶对牛血液蛋白的酶解效果研究[J].现代畜牧兽医,2025,(4):28-32,5.基金项目
中央高校基本科研业务费项目(31920240110) （31920240110）
2024年度实验室开放项目(SYSKF-2024072) （SYSKF-2024072）
