现代畜牧兽医Issue(4)：33-37,5.DOI:10.20154/j.cnki.issn1672-9692.2025.04.007
猪流行性腹泻病毒在反应器培养过程中提高病毒滴度的方法研究
Study on the method of increasing viral titer of porcine epidemic diarrhea virus in the process of reactor culture
赵晓英 1寇春 1王娟 1孙聪 1张华 1杜久斌 1贺笋1
作者信息
- 1. 天康制药股份有限公司,江苏 苏州 215100
- 折叠
摘要
Abstract
The experiment aimed to investigate the effect of culture medium osmotic pressure on the expression of porcine epidemic diarrhea virus(PEDV).PEDV was inoculated into serum-free medium containing ST suspension cells,and sodium chloride was added to adjust the osmotic pressure of the cell culture medium during the viral expression phase.The effects of varying osmotic pressure conditions on cell growth,metabolic activity,and viral titer were evaluated.Results showed that maintaining the osmotic pressure of the culture medium at(360±10)mOsm/kg during viral expression by sodium chloride supplementation increased the viral titer from 7.50 Log TCID50/mL to 8.80 Log TCID50/mL,significantly enhancing the harvested PEDV antigen or viral seed titer.This study demonstrates that regulating the osmotic pressure of the culture medium can markedly improve PEDV viral titer during expression.关键词
猪流行性腹泻病毒/猪睾丸(ST)悬浮细胞/渗透压/病毒滴度Key words
Porcine epidemic diarrhea virus/ST suspension cells/Osmotic pressure/Viral titer分类
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赵晓英,寇春,王娟,孙聪,张华,杜久斌,贺笋..猪流行性腹泻病毒在反应器培养过程中提高病毒滴度的方法研究[J].现代畜牧兽医,2025,(4):33-37,5.
