煤炭转化2025，Vol.48Issue(3)：92-104,13.DOI:10.19726/j.cnki.ebcc.202503011
不同细菌对义马低阶煤降解效果及降解条件优化
Degradation effect of different bacteria on Yima low-rank coal and optimization of degradation conditions
摘要
Abstract
To obtain efficient bacteria for low-rank coal conversion,three strains were iso-lated from decayed wood,supplemented with an additional three strains from coal mines preserved in the laboratory.Subsequently,two dominant degrading strains,Pantoea ananatis and Bacillus subti-lis,were identified based on their degradation abilities regarding Yima low-rank coal.Single-factor experiments combined with responsesurface methodology were employed to optimize the degradation conditions of the two bacterial strains on Yima low-rank coal,while infrared spectroscopy was utilized to characterize both the raw coal and residual coal.The results indicate that the optimal conditions for the degradation of low-rank coal by Pantoea ananatis are 3%bacterial inoculum,16.8 mg/mL coal slurry concentration,182 r/min,pH 8.65 and 32℃.Conversely,the optimal conditions for Bacillus subtilis are determined as 2%bacterial inoculum,21.4 mg/mL coal slurry concentration of,184 r/min,pH 7.02 and 30℃.The coal degradation ratios observed in the validation experiments are 23.6%and 22.7%,respectively.Following the bacterial degradation of the coal samples,the absorption peaks for aromatic hydrocarbons at C=C and C=O exhibited the most substantial changes.Both bacterial strains demonstrate a superior capacity to degrade the hydroxyl groups present in the coal samples and also affect the coal's aromatic structure.Moreover,Pantoea ananatis exhibits a greater efficacy in degrading low-rank coal compared to Bacillus subtilis.关键词
菠萝泛菌/枯草芽孢杆菌/低阶煤/生物降解/响应面法Key words
Pantoea ananatis/Bacillus subtilis/low-rank coal/biodegradation/response surface methodology分类
化学化工引用本文复制引用
刘建民,彭洁,连梦思,王明仕,陈林勇,任恒星..不同细菌对义马低阶煤降解效果及降解条件优化[J].煤炭转化,2025,48(3):92-104,13.基金项目
国家自然科学基金重点项目(42230804)和河南省科技攻关项目(242102320191). （42230804）
